陆地细菌分离物产生表面活性生物表面活性剂的能力:遗传鉴定,生化和形态方法
Ahmed Mohy Eldin1, Nermeen Hossam2
1Department of Soil Microbiology, Soils, Water and Environmental Research Institute (SWERI), Agricultural Research Center (ARC), Giza, 12619, Egypt. ameaefe2006@yahoo.com.
概括
一种被称为Brucella anthropi的细菌分离物有效地产生生物表面活性剂,显著降低表面张力和乳化油. 华介质在优化生物表面活性剂生产方面被证明是最有效的.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 表面化学 表面化学
背景情况:
- 生物活性剂是具有多样化工业应用的两性化合物.
- 生物表面活性剂的高效微生物生产对于可持续的过程至关重要.
- 鉴定新型生物表面活性剂产生细菌的特征对于扩大它们的实用性至关重要.
研究的目的:
- 为了评估细菌分离物的生物表面活性剂生产效率.
- 为了识别细菌菌株并描述其生物表面活性剂特性.
- 为了确定最佳的培养基以提高生物表面活性剂产量.
主要方法:
- 使用九种分析方法测量了表面张力减小.
- 乳化活性测定 (E24%) 和CTAB测试.
- 碳化合物降解,脂酶生成和血液溶解活性测定.
- 通过形态,生化测试和16S rRNA基因测序进行细菌鉴定.
- 在五种不同的培养介质中对生物表面活性剂生产的比较分析.
主要成果:
- 细菌分离物13(1) 显示显著降低表面张力 (42.84%) 和乳化 (41.07%).
- 鉴定为Brucella anthropi的分离物对阳离子表面活性剂和碳化合物降解呈阳性结果.
- 华媒产生了最高的生物表面活性剂产量,表面张力减少了45.81%,表面活性为20.30 mN m-1 .
结论:
- 布鲁塞拉 (Brucella anthropi) 是一个高效的生物表面活性剂生产者,具有有前途的表面活性.
- 华介质被确定为最适合的基质,可以优化B. anthropi.的生物表面活性剂生产.
- 这些发现支持微生物生物表面活性剂在各种工业应用中的潜力.
相关概念视频
Genetics of Speciation
21.9K
Speciation is the evolutionary process resulting in the formation of new, distinct species—groups of reproductively isolated populations.
21.9K
Types of Genetic Transfer Between Organisms
31.1K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
31.1K
What is Genetic Engineering?
80.4K
Overview
80.4K
Prokaryotic Transcriptional Activators and Repressors
25.6K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
25.6K
Bacterial Signaling
41.3K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
41.3K
What is Population Genetics?
65.0K
A population is composed of members of the same species that simultaneously live and interact in the same area. When individuals in a population breed, they pass down their genes to their offspring. Many of these genes are polymorphic, meaning that they occur in multiple variants. Such variations of a gene are referred to as alleles. The collective set of all the alleles within a population is known as the gene pool.
65.0K


