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在长期冷藏期间对 rhesus 和 cynomolgus 的全血进行评估
Alejandra L Lorenzen1, Susan M Shea2,3, Leslie E Neidert1
1Naval Medical Research Unit San Antonio, JBSA-Fort Sam Houston, TX.
Shock (Augusta, Ga.)
|February 11, 2026
概括
非人类灵长类 (NHP) 的冷储全血 (WB) 显示稳定的红细胞,但在7天后静血功能下降. 这项研究以使用存储的异构WB的创伤复苏模型为基础.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 创伤研究 创伤研究
- 储存血液 储存血液 储存血液
背景情况:
- 冷储全血 (WB) 越来越多地用于大规模出血复苏.
- 非人类灵长类动物 (NHP) 是血液静止研究的关键临床前模型,因为它们与人类有生理上的相似性.
- 目前的NHP创伤模型经常使用自主棚WB,不能完全复制人类临床场景.
研究的目的:
- 评估长时间4°C储存对NHP WB的影响.
- 在 rhesus 和 cynomolgus WB. 的储存损伤的特征.
- 评估在NHP复苏模型中使用储存的异质WB的可行性.
主要方法:
- 从 rhesus 和 cynomolgus 身上收集了 WB,并在 CPDA-1 中以 4°C 的温度储存.
- 生物化学,血液学和静血学参数每天测定4天,然后每周测定至28天.
- 数据使用混合效应分析和多重比较来分析.
主要成果:
- 红细胞计数,血红蛋白和血红素在28天的储存时间内保持稳定.
- 乳酸盐和水平显著增加,而和pH值显著下降.
- 从第7天起,两种物种的血静功能显著下降.
结论:
- 建立了一种可行的制造存储NHP WB的方法.
- 该研究描述了NHP WB的储存损伤,没有观察到明显的物种间差异.
- 在未来的NHP复苏研究中利用存储的异构WB可以提高翻译相关性.
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