一次性使用的Ambu aScope基础的微侵袭性支气管支气管支气管洗采集在猪中的协议
Piyush Baindara1, Dinata Roy1, Addison K Byrne1
1Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA; National Swine Testing Center, University of Missouri, Columbia, MO 65211, USA.
STAR protocols
|February 11, 2026
概括
这项研究详细介绍了一项采集来自猪的支气管洗 (BAL) 样本的协议,使用一次性使用的AmbuaScope. 该方法确保了用于猪模型的呼吸研究和治疗测试的高质量样本.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 呼吸系统生理学 呼吸系统生理学
- 动物研究方法 动物研究方法
背景情况:
- 支气管支气管洗 (BAL) 对于呼吸系统研究至关重要.
- 为了在动物模型中进行一致的样本采集,需要标准化的协议.
- 猪是人类呼吸道疾病的宝贵模型.
研究的目的:
- 提出一种标准化方案,用于气泡洗 (BAL) 采集活猪的样本.
- 详细说明使用一次性使用的AmbuaScope用于BAL程序.
- 确保在呼吸系统研究中进行下游分析的高质量的BAL样本.
主要方法:
- 描述了使用一次性Ambu aScope进行活猪制备和支气管支气管洗 (BAL) 的协议.
- 提供了详细的样本处理和下游分析步骤.
- 该协议强调可重复结果的一致性和质量.
主要成果:
- 提出的协议允许可靠地从猪中采集BAL样本.
- 使用一次性使用的Ambu aScope使程序更容易.
- 该方法支持用于研究的一致,高质量的样本采集.
结论:
- 该协议提供了一种可复制的方法,用于从猪中获取BAL样本.
- 它适用于各种呼吸道研究领域,包括炎症研究.
- 该方法支持使用猪模型来评估新型呼吸道疗法.
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