光遗传控制过渡到变形的过程
Changyuan Wang1, Denis F Faerberg2, Yuka Sekine3
1Department of Physics, Princeton University, Princeton, NJ 08544.
概括
我们使用光遗传学开发了一种用于Drosophila的转化承诺的预测模型. 这种系统识别方法准确地预测了发育过渡,为类似的过程提供了洞察力,例如哺乳动物的青春期.
科学领域:
- 发展生物学 发展生物学
- 系统生物学 系统生物学
- 神经内分泌学神经内分泌学
背景情况:
- 在Drosophila中,转化承诺是一种由神经内分泌回路调节的关键发育过渡.
- 之前的研究使用了静态遗传干扰和饥饿,限制了这些电路的动态分析.
- 了解青少年到成人的过渡至关重要,与哺乳动物的青春期相似.
研究的目的:
- 在整个生物体尺度上应用系统识别以建模Drosophila变形承诺.
- 开发一个神经内分泌控制变形的预测数学模型.
- 利用光遗传学来精确,动态地扰乱关键信号节点.
主要方法:
- 在Drosophila幼虫中采用光遗传方法来扰乱一个关键的内分泌腺信号节点.
- 应用系统识别技术,从幼虫对干扰的反应推断参数.
- 基于实验数据开发并验证了一种紧的数学模型.
主要成果:
- 成功建立了Drosophila变形承诺的预测数学模型.
- 使用推断的模型参数,证明了幼虫承诺的准确预测.
- 在全生物体研究中展示了光遗传学对动态扰动的有效性.
结论:
- 系统识别是一种强大的方法,可以在生物水平上建模复杂的发育过渡.
- 开发的模型准确地预测了Drosophila的转化承诺.
- 这项工作为从青少年到成人过渡的定量研究提供了框架,包括哺乳动物的青春期.
相关概念视频
Phase Transitions
23.3K
Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to...
23.3K
Properties of Transition Metals
30.1K
Transition metals are defined as those elements that have partially filled d orbitals. As shown in Figure 1, the d-block elements in groups 3–12 are transition elements. The f-block elements, also called inner transition metals (the lanthanides and actinides), also meet this criterion because the d orbital is partially occupied before the f orbitals.
30.1K
Cooperative Allosteric Transitions
8.9K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
8.9K
Cooperative Allosteric Transitions
2.7K
2.7K
Cooperative Allosteric Transitions
3.1K
3.1K
Phase Transitions: Vaporization and Condensation
21.6K
The physical form of a substance changes on changing its temperature. For example, raising the temperature of a liquid causes the liquid to vaporize (convert into vapor). The process is called vaporization—a surface phenomenon. Vaporization occurs when the thermal motion of the molecules overcome the intermolecular forces, and the molecules (at the surface) escape into the gaseous state. When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase molecules...
21.6K


