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Updated: Feb 13, 2026

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通过TD-198946调节IPSC衍生的NCMSC细胞状态,增强了无支架软骨生物制造
Anna Nakamura1, Denise Zujur2, Toshihiro Nonaka3
1Center for Regenerative Medicine Research, Faculty of Medicine, Saga University, Honjomachi 1, Saga, 840-8502, Japan.
Biofabrication
|February 11, 2026
概括
使用TD-198946预先调配诱导多能干细胞 (iPSCs),可以提高无支架3D生物打印的细胞质量. 这改善了软骨结构的大小,强度和糖氨基酸沉积,以更好地修复组织.
科学领域:
- 再生医学是一种再生医学.
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
背景情况:
- 无脚手架的生物制造通过避免人工材料提供了有前途的软骨修复.
- 可复制的3D生物打印依赖于坚固的细胞群,特别是来自扩展诱导多能干细胞 (iPSC) 衍生的介质干细胞 (iNCMSCs).
- 重复的单层扩展可能会损害iNCMSC的质量,影响生物打印结果.
研究的目的:
- 调查TD-198946预条件是否可以在扩展过程中稳定iNCMSC质量.
- 评估TD-198946对细胞增殖,细胞循环和N-cadherin表达的影响.
- 评估TD-198946预条件对无脚手架3D生物打印和结构成熟的影响.
主要方法:
- iNCMSC的扩展有或没有TD-198946预先条件.
- 分析了细胞增殖 (MTT测定),细胞周期 (G1停止) 和N-cadherin表达.
- 使用NOTCH3信号抑制器DAPT来探测TD-198946的机制.
- TD-198946预先调节的细胞被用于无脚手架的3D生物打印.
- 建筑物被评估为球形尺寸,糖氨基甘油 (GAG) 沉积和机械强度.
主要成果:
- TD-198946 预先条件增加了iNCMSC的增殖和改善细胞循环的进展,效应由NOTCH3信号介导.
- 通过TD-198946对N-cadherin表达进行了上调,促进了球状凝聚力.
- 使用TD-198946原始化的无脚手架3D生物打印,导致构造尺寸,GAG含量和机械强度 (最佳在50nM) 的剂量依赖性增加.
- 较高的TD-198946度 (100nM) 对细胞外基质的产生和机械性能产生了负面影响.
结论:
- TD-198946预条件增强了扩大的iNCMSC种群的强度.
- 这种稳定性提高了无支架3D生物打印的一致性和质量.
- 优化的TD-198946治疗促进了对潜在的治疗应用更好的软骨结构成熟.
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