从使用HPLC-HRMS/MS和电化学探测器的AAPH反应中发现红菜类安托西亚宁的结构-活性关系
Claudia Lombroni1, Lucrezia Angeli2, Matteo Scampicchio2
1University of Turin, Department of Agricultural, Forest and Food Sciences, 10095 Grugliasco (TO), Italy; Free University of Bozen-Bolzano, Faculty of Agricultural, Environmental and Food Sciences, 39100 Bolzano (BZ), Italy.
红菜的安东基氨酸显示出强大的抗氧化活性,具有增强激素清理的特定结构. 这项研究整合了动力学和电化学方法,以实现对食品中抗氧化剂功能的现实评估.
科学领域:
- 食品化学 食品化学
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 安西安素是红菜中发现的关键的多抗氧化剂.
- 以前的研究经常使用单独的测试来评估抗氧化剂活性.
- 有限的研究整合了动力学和电化学分析,以进行全面的分析.
研究的目的:
- 用多方法方法评估红菜安东的抗氧化活性.
- 研究结构特征对抗氧化能力的影响.
- 提供复杂食品矩阵中抗氧化剂功能的机制性评估.
主要方法:
- 高分辨率HPLC-MS/MS用于安托西亚尼的识别和量化.
- 停止流动的DPPH动力测定和AAPH启动的降解测定,以测量激素清除能力.
- 使用HPLC-DAD-CAD进行电化学分析 (半波电位测定).
主要成果:
- 鉴定和量化了13种基于化的素衍生物.
- 氨基-3-(disinapoyl) -二甲化-5-葡萄糖化 (Cy3(disin) diG5G) 显示了最高的降解率.
- 具有较低半波电位 (E1/2) 的化合物表现出更高的反应性,其中Cy3(disin) diG5G具有最低的E1/2 (+525 mV).
结论:
- 像乙化和糖化这样的结构特征显著地影响了氨酸的氧化还原潜力和降解动力学.
- 综合动力学和电化学方法为抗氧化活性提供了更加机械和现实的评估.
- 这些发现有助于理解食物中的多抗氧化剂的结构-活性关系.
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