工程设计的无载荷纳米粒子诱用于细胞对巨细胞的调制
Peyton M Panovich1, Aditi Ganesan1, Arianna I Markey1
1Department of Biomedical Engineering and Critical Care Medicine Division, University of Michigan Medical School, Ann Arbor, MI 48109, United States of America.
概括
无载荷的聚乳糖酸纳米颗粒 (NP) 充当诱,减少巨细胞吸收治疗性NP. 这一策略降低了肝脏的积累,并提高了纳米药物的肺部输送.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 免疫学 免疫学 免疫学
背景情况:
- 聚合物纳米粒子 (NP) 对非病毒基因疗法具有前景.
- 一个主要的挑战是它们被单核细胞系统 (MPS) 快速清除,导致它们在肝脏和脏积累.
- 这限制了通过静脉注射系统输送NP的治疗疗效.
研究的目的:
- 调查使用无货物聚乳糖糖酸 (PLGA) NP作为"诱"预处理来规避MPS清除.
- 为了确定诱惑性NP是否可以调节巨细胞的细胞活动,并改善随后给药的治疗性NP的输送.
- 探索影响诱NP疗效的因素,包括尺寸,表面活性成分和时间.
主要方法:
- 在体外研究中,使用流细胞计和光显微镜来评估诱NP预处理后巨细胞对治疗性NP的吸收.
- 在体内研究评估治疗NP的生物分布后诱NP的预给药.
- 系统地改变诱NP特征 (大小,表面活性剂,时间) 以优化细胞调节.
主要成果:
- 无载荷的PLGA诱NP显著减少了巨细胞在体外吸收治疗性NP的数量.
- 诱惑性NP的预先使用导致治疗性NP在肝脏中的积累减少,并在体内肺部的沉积增加.
- 诱惑NP的有效性受到尺寸,表面活性剂成分和预处理时间的影响.
结论:
- 用无载荷PLGA诱NP进行预处理是一种有效的策略,可以减少治疗性NP的MPS介导清除.
- 这种方法有可能提高纳米药物的输送,以向肝脏和脏以外的组织.
- 诱NP技术为改善纳米医学治疗的治疗指数和疗效提供了一个有前途的途径.
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