具有pH响应性和可回收的同质碳酸无水酶与24聚合物融合蛋白:简单的制备和增强的催化性能
Chun Yang1, Zicheng Wu1, Yaxin Chen1
1Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, Fujian Province, PR China.
Bioresource technology
|February 11, 2026
概括
一种基于的新策略使可利用酶 - 费里融合蛋白的可回收均生物催化剂成为可能. 这种方法简化了酶制备和循环利用,同时提高了稳定性和性能.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 蛋白质工程和设计
- 绿色化学和可持续的过程
背景情况:
- 酶是有效的生物催化剂,但在制备,稳定性和可重复使用性方面面临挑战.
- 现有的化学或基因改造为同时改进提供了有限的解决方案.
- 需要综合战略来克服酶应用中的这些局限性.
研究的目的:
- 开发一种基于的新策略,用于制造可回收的同质生物催化剂.
- 为了提高酶的稳定性,性能,并简化制备和回收过程.
- 为了研究酶 - 费里丁融合蛋白的pH响应行为.
主要方法:
- 工程化祖先碳酸无水酶 (AncCA19) 与铁素 (AncCA19F) 融合,形成酶 - 铁素融合蛋白 (EFFP).
- 自组装EFFPs成微米大小的颗粒 (msAncCA19F) 通过离心法简化净化.
- pH诱导的msAncCA19F的可逆组装/拆卸到纳米尺寸的同质催化剂 (nsAncCA19F) 和背后.
主要成果:
- 精简了酶 - 费里融合蛋白的净化和提高了产量.
- 增强的酶性能:人工海水中半衰期增加了9倍,MDEA中增加了2倍.
- 固定生物催化剂的高可回收性,在10个循环后<10%的活性损失.
结论:
- 基于的策略使得时间有效,可扩展的酶制备和简单的回收利用.
- 开发的生物催化剂通过pH响应组件表现出更好的稳定性和性能.
- 这种方法为克服酶应用中的局限性提供了一个有希望的解决方案.
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