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工程Corynebacterium glutamicum通过提高产品耐受性和排泄物运输来生产1,5-二胺
Longfei Song1, Landong Zhang2, Jia Liu1
1School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.
Bioresource technology
|February 11, 2026
概括
这项研究设计了Corynebacterium glutamicum来克服1,5-Pentanediamine (PDA) 细胞毒性,以改善微生物的产生. 双重耐受性策略提高了从葡萄糖产生的PDA产量,达到130g/L.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 1,5-丁胺 (PDA) 是各种行业的关键单体,但其微生物产量受到细胞毒性限制,这是由于快速的细胞内积累.
- 高的细胞内和细胞外压力限制了PDA的高效发酵.
研究的目的:
- 开发一种强大的微生物菌株,用于高水平的1,5-丁胺 (PDA) 生产.
- 设计Corynebacterium glutamicum以提高对细胞内和细胞外PDA毒性的耐受性.
主要方法:
- 在Corynebacterium glutamicum中实施了双重耐受性工程策略.
- 确定了与膜相关的耐受性目标,并利用适应性实验室进化 (ALE) 来发现细胞外耐受性的cgl1283决定因素.
- 设计了一种高活性CgmA流量输送器突变,以减少细胞内PDA积累.
主要成果:
- 通过ALE和cgl1283.3的鉴定,通过ALE实现了细胞外PDA耐受性的显著改善.
- 通过改造CgmA流量载体,降低了细胞内PDA水平.
- 在5L生物反应器中优化了该过程,从葡萄糖中产生130g/L的PDA,产量为0.40g/g,生产率为3.94g/L/h.
结论:
- 通过整合细胞内和细胞外耐受性工程,建立了对微生物产生有毒化学品的综合框架.
- 证明了在生产PDA等高价值化学品时克服细胞毒性的有效策略.
- 工程菌株LF6.1显示出对工业规模的1,5-Pentanediamine微生物合成的希望.
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