用黄金纳米颗粒优化阿普坦酶触发的杂化连锁反应,以快速视觉检测ATP
Shengli Zhou1, Hiroko Fukaya1, Shunsuke Watanuki1
1Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan. ckeitaro@mail.ecc.u-tokyo.ac.jp.
Analytical methods : advancing methods and applications
|February 11, 2026
概括
这项研究开发了一种快速的,可视化的腺三酸盐 (ATP) 检测方法,使用DNA吸收体和金纳米粒子. 优化的测定只需15分钟就能实现视觉ATP检测,比以前的技术要快得多.
科学领域:
- 生物技术是生物技术.
- 纳米技术纳米技术
- 分析化学 分析化学
背景情况:
- 快速,简单,可视检测三氨酸 (ATP) 对于诊断和环境监测至关重要.
- 目前使用金纳米颗粒 (AuNPs) 的DNA体触发混合链反应 (HCR) 试验很慢,通常超过130分钟.
研究的目的:
- 通过优化aptamer触发的HCR和AuNP集成来开发ATP的快速视觉检测平台.
- 为了加速HCR动力学和改善AuNP混合条件,以更快地检测ATP.
主要方法:
- 系统地调查和优化HCR参数,包括单链DNA (ssDNA) 度和离子 (Mg2+) 存在.
- 优化HCR产品和AuNP解决方案之间的混合比,以平衡HCR速度和AuNP稳定性.
- 对视觉ATP检测优化试验的评估.
主要成果:
- HCR动力学显著加速,通过优化ssDNA和Mg2+度,在60分钟内获得24小时化结果.
- 优化的混合条件使得在15分钟的总测定时间内,可以快速视觉检测100μM ATP.
- 与现有的ATP检测试验相比,开发的方法代表了相当大的加速.
结论:
- 经过优化,由阿普坦酶触发的HCR-AuNP平台为ATP提供了显著更快的视觉检测方法.
- 这种快速测试对临床诊断和环境监测应用具有前景.
- 未来的工作重点是通过AuNP参数微调和盐聚合增强剂来提高灵敏度.
相关概念视频
ATP Yield
79.1K
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle).
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
79.1K
Hydrolysis of ATP
81.9K
The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
81.9K
Electron Transport Chains
113.1K
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
The ETC is comprised of...
113.1K
Free-Radical Chain Reaction and Polymerization of Alkenes
9.7K
The conversion of alkenes to macromolecules called polymers is a reaction of high commercial importance. The structure of the polymer is defined by a repeating unit, while the terminal groups are considered insignificant. The average degree of polymerization represents the number of repeating units in the polymer molecule and is denoted by the subscript n.
9.7K
Hybrid Zones
22.0K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
22.0K
Hybridization of Atomic Orbitals I
67.8K
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
67.8K


