使用Engram进行生物信息的多通道基因组记录
Jenny F Nathans1,2,3, Troy A McDiarmid4,5, Wei Chen6,7,8
1Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Nature protocols
|February 11, 2026
概括
本研究介绍了增强器介导的多重体活动基因组记录 (ENGRAM) 系统,用于稳定的分子记录. 恩格拉姆提供了一种可扩展的方法,用于使用DNA测序分析cis-regulatory元素活动.
科学领域:
- 合成生物学 合成生物学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 分子记录使生物过程的时间测量成为可能.
- 现有的方法在可扩展性和多重化方面存在局限性.
- 控制基因表达的Cis-regulatory元素 (CREs) 是研究的关键目标.
研究的目的:
- 为增强器介导的多重体活动基因组记录 (ENGRAM) 系统提供详细的协议.
- 概述Engram的设计考虑,优势,局限性和应用.
- 为了使CREs的高通量选和多重信号记录成为可能.
主要方法:
- 恩格拉姆利用主要编辑介导的插入来创建CRE活动的稳定基因组记录.
- 该系统设计用于固有的多重复合,记录多达256个不同的CRE活动,使用四个基对插入.
- 与DNA打字机的兼容性有助于捕获信号顺序.
主要成果:
- 该协议详细说明了ENGRAM的实验程序和数据分析.
- 恩格拉姆展示了用于CRE活动记录的多重复合能力.
- 该系统与DNA Typewriter兼容,用于时间信号捕获.
结论:
- 恩格拉姆为CRE活动的分子记录提供了一个强大而可扩展的平台.
- 该技术可促进高通量查和多重生物信号分析.
- 对于具有基本分子生物学技能的研究人员来说,ENGRAM实验在5-6周内是可行的.
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