对非洲猪瘟病毒抗体检测的快速和视觉双色侧流免疫试验
Jie Chen1,2, Zhengwang Shi1,2, Yi Ru1,2
1State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Applied microbiology and biotechnology
|February 11, 2026
概括
一种新的双色侧流免疫试验 (LFIA) 可以快速准确地检测非洲猪瘟病毒 (ASFV) 抗体. 这种具有成本效益的方法为临床检测提供了高灵敏度和稳定性,有助于疾病管理.
科学领域:
- 兽医诊断 兽医诊断 兽医诊断 兽医诊断
- 免疫学检测 免疫学检测
- 传染病检测检测 传染病检测
背景情况:
- 非洲猪瘟 (ASF) 是一种高度致命的猪病,影响全球猪产量和经济稳定.
- 目前的ASF管理依赖于诊断和杀,因为没有疫苗和有效的治疗方法.
- 对于ASF而言,有必要提供灵敏,快速和简单的诊断工具.
研究的目的:
- 开发一种基于乳微球 (LM) 的新型双色侧流免疫试验 (LFIA) 用于检测非洲猪瘟病毒 (ASFV) 抗体.
- 评估开发的LFIA的灵敏度,特异性,稳定性和可重复性.
- 评估该试验是否适用于ASF诊断的临床检测 (POCT).
主要方法:
- 结合ASFV p72抗原与红色LM和IgY与蓝色LM进行双色检测.
- 开发一种侧流免疫试验格式,可在15分钟内对ASFV抗体进行视觉检测.
- 使用标准血清,常见的猪病原体,临床样本的验证,并与商业ELISA套件进行比较.
主要成果:
- 双色LM-LFIA在15分钟内可视检测到ASFV抗体,没有交叉反应.
- 该试验的灵敏度为1:1024,与商业ELISA套件相比,临床样品的卡帕值为0.955.
- 在各种储存温度下观察到出色的稳定性,通过内部和测试间的可变性测试证实了高的重复性.
结论:
- 开发的双色LM-LFIA是一种敏感,特异,稳定和快速的ASFV抗体检测方法.
- 该测试的视觉直观性和易用性使其成为ASF管理中的治疗点测试 (POCT) 的理想选择.
- 这种创新的LFIA提供了一种具有成本效益的解决方案,以改善早期检测和控制非洲猪瘟.
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