Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
Orthogonal Trajectories01:26

Orthogonal Trajectories

72
Orthogonal trajectories describe the geometric relationship between two families of curves that intersect each other at right angles. One illustrative case involves a family of parabolas that open sideways along the x-axis. These curves share a common shape but differ by a scaling parameter, resulting in a set of curves that all pass through the origin and widen at different rates.Determining Orthogonal TrajectoriesTo identify the orthogonal trajectories for these parabolas, the first step...
72
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

6.8K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
6.8K
The Movement of Organelles and Vesicles01:43

The Movement of Organelles and Vesicles

6.6K
In eukaryotic cells,  cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
6.6K
Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)01:16

Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)

3.2K
Ring-opening metathesis polymerization or ROMP involves strained cycloalkenes as starting materials. The mechanism of ROMP proceeds by reacting cycloalkene with Grubbs catalyst to give metallacyclobutane intermediate which undergoes a ring-opening reaction to form new carbene. The new carbene reacts with another molecule of cycloalkene. Repetition of these steps leads to the formation of an unsaturated open-chain polymer product. All these steps are reversible, however, relieving the ring...
3.2K
Actin Polymerization01:42

Actin Polymerization

8.6K
Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
8.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Clinical performance of two lithium disilicate CAD/CAM materials in posterior Class II inlay restorations: a 48-month randomised split-mouth clinical trial.

Journal of dentistry·2026
Same author

Outcomes and Prognostic Factors of Auto-Transplanted Immature Third Molars: A 15-Year Retrospective Cohort Study.

International dental journal·2026
Same author

Clinical Outcome of Replanted Avulsed Anterior Teeth: A Retrospective Cohort Study.

Journal of endodontics·2026
Same author

Effects of repeated preheating on polymerization shrinkage kinetics and Vickers microhardness of resin composites: an in vitro study.

BMC oral health·2026
Same author

Diode-pumped prism-free Ti:sapphire oscillator delivering 10-fs pulses.

Optics express·2026
Same author

Prediction of delirium in trauma patients using interpretable machine learning.

Scientific reports·2026

相关实验视频

Updated: Feb 13, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K

有机细胞定位诱导的生物正交聚合 (OLIBOP) 用于光稳定超分辨率活细胞成像.

Gaeun Park1,2, Sangpil Kim1, Dohyun Kim1

  • 1Department of Chemistry, College of Natural Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.

Advanced healthcare materials
|February 11, 2026
PubMed
概括

研究人员开发了一种新方法,可以在特定细胞部位内创建光稳定的光探针,从而在没有光漂白的情况下长期对线粒体动力学进行活细胞成像.

关键词:
聚合引发的排放量 聚合引发的排放量生物对等反应的生物对等反应在现场聚合.活细胞成像成像技术有机器人 有机器人

更多相关视频

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
12:28

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Published on: November 10, 2017

9.9K

相关实验视频

Last Updated: Feb 13, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.3K
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
12:28

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Published on: November 10, 2017

9.9K

科学领域:

  • 生物医学工程 生物医学工程
  • 化学生物学 化学生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 光漂白限制了动态生物过程的长期活细胞成像.
  • 聚合聚合诱导排放光原体 (AIEgens) 提供光稳定性,但通常局限于溶解体.
  • 需要具有更广泛的亚细胞可访问性和更好的光稳定性的探测器.

研究的目的:

  • 开发一种新的方法,用于在特定有机体内合成光稳定的聚合物光探针.
  • 克服当前探针的局限性,特别是溶酶体局部化和光漂白.
  • 为了实现细胞动态的实时,长期成像,特别是线粒体功能.

主要方法:

  • 使用了Organelle定位诱导的生物对角聚合 (OLIBOP) 策略.
  • 一个小分子前体 (1-AIE) 被设计为具有AIE特性,线粒体向部分和生物对等的凝聚组.
  • 线粒体局部化和现场聚合是由GSH响应性二硫化键触发的,使用相位-FLIM可视化.

主要成果:

  • 设计的前体 (1-AIE) 在特定的线粒体位点成功地在现场聚合.
  • 由此产生的局部形成的聚-AIEgen 呈现出显著增强的光强度和更长的光寿命.
  • 特殊的光稳定性允许在长时间内对线粒体动态进行前所未有的实时跟踪.

结论:

  • OLIBOP提供了一种突破性的方法,以克服探头交付和稳定性之间的权衡.
  • 这种方法可以在体内制造光稳定的聚合物探头,从而提高生物相容性.
  • 开发的探测器可提供高分辨率,长期活细胞成像细胞过程,特别是线粒体动力学.