一种染色学方法,用于同时量化人类血样本中的多种抗凝剂
Federica Guidetti1, Sara R Fernandes1, Luisa Barreiros1,2
1LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal.
一种新的HPLC方法允许在血中快速,同时量化四种关键抗凝剂. 这种方法有助于监测华法林,达比加特兰,里瓦罗克萨班和阿皮克萨班,特别是在特殊的临床情况下.
科学领域:
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 抗凝剂对于治疗血栓栓塞性疾病至关重要.
- 华法林需要严格监测,因为其治疗窗口狭窄,并有不良事件的风险.
- 直接口服抗凝剂 (DOAC) 提供了更好的安全性,但在特定的临床场景中可能需要监测.
研究的目的:
- 开发和验证一种高性能液体染色学 (HPLC) 方法,用于同时量化人血中华法林,达比加特兰,里瓦罗克萨班和阿皮克萨班的含量.
- 建立一个可靠的分析工具,用于监测这些抗凝剂在临床实践.
主要方法:
- 使用HPLC与UV/Vis检测同时量化四种抗凝剂.
- 方法开发涉及优化柱子类型 (单体柱),化条件 (梯度) 和注射体积.
- 验证包括评估选择性,线性,准确性,精度,检测极限 (LOD) 和量化极限 (LOQ).
主要成果:
- 开发的HPLC方法在12分钟内实现了四种抗凝剂的有效分离.
- 该方法表现出极好的线性,准确度 (85.7%-115.0%) 和精度 (CV ≤9.4%).
- 分析物的稳定性在室温下在24小时内得到证实,在结解周期后,对达比加特兰发现了轻微的稳定性问题.
结论:
- 成功开发了一种快速,选择性和验证的HPLC方法,用于同时量化人体血中的四种主要抗凝剂.
- 该方法为治疗药物监测提供了宝贵的工具,特别是在复杂的临床情况下,需要精确的抗凝剂水平评估.
更多相关视频
07:08Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood
Published on: September 5, 2017
08:29Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
Published on: September 9, 2020
相关概念视频
Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...
Chromatographic Resolution
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
Chromatographic Methods: Terminology
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Anticoagulant Drugs: Low-Molecular-Weight Heparins
