通过突变性和源优化增强Monascus purpureus中的Monacolin K生产
Chen Yang1, Yang Yajing1, Hong Housheng1
1Nanjing Tech University, Nanjing, China.
Journal of basic microbiology
|February 12, 2026
概括
研究人员增强了Monascus真菌的功能.
科学领域:
- 生物技术和微生物发酵
- 代谢工程是代谢工程.
- 自然产品的合成自然产品的合成
背景情况:
- 蒙纳斯菌对于生产Monacolin K (MK) 很有价值,这是一个因其降脂作用而闻名的化合物.
- 优化MK产量对于其治疗应用至关重要.
- 固态发酵是一种常见的真菌培养方法.
研究的目的:
- 通过突变发生和营养补充,改善Monascus真菌中Monacolin K (MK) 的产量.
- 调查增强MK生产背后的代谢机制.
- 开发一种高产的Monascus菌株,用于高效的MK生物合成.
主要方法:
- 大气和室温等离子体 (ARTP) 突变发生被用来产生突变菌株.
- 测试了L-氨酸补充剂,以提高固态发酵中的MK产量.
- 使用非向代谢学来分析代谢途径的变化.
- 使用已建立的分析方法进行了MK量化.
主要成果:
- 一种被指定为C-23的突变菌株证明了MK生产能力的提高.
- 补充L-氨酸显著增加了MK产量97.3%,达到9.45 mg/g.
- 代谢分析显示,L-氨酸调节涉及氨基酸,辅因子,碳水化合物和脂质代谢的途径.
- 这些代谢变化为MK过度产生的机制提供了洞察力.
结论:
- 结合ARTP突变发生和L-氨酸补充的结合方法有效地提高了Monascus中的MK产量.
- 氨酸在调节对MK生物合成至关重要的代谢途径中起着关键作用.
- 这项研究提供了一种战略方法,用于开发用于Monacolin K生产的优质Monascus菌株.
- 这些发现有助于了解真菌中自然产品合成的代谢调节.
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