单核转录组揭示了细胞异质性和转录性反应的热应激在骨肌肉中的反应
Ziyin Han1,2, Li Chen3,4, Zhiyu Lei1
1State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Journal of cachexia, sarcopenia and muscle
|February 12, 2026
概括
热应激会损伤骨肌肉,导致细胞亡和氧化应激. 这项研究揭示了单细胞转录水平上热引起的肌肉损伤和修复的分子机制,确定肌纤维和IIb型肌核是最敏感的.
科学领域:
- 肌肉生理学 肌肉生理学
- 分子生物学分子生物学
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 热应激会导致骨肌损伤,其特点是亡,氧化应激和自.
- 了解热引起的肌肉损伤的分子基础,特别是在单细胞转录水平上,仍然有限.
研究的目的:
- 为了研究潜在的分子机制热应激诱导的骨肌肉损伤和修复.
- 以单细胞分辨率构建暴露于热量的骨肌肉的综合转录组数据集.
主要方法:
- 从小鼠的骨肌肉暴露在不同的热应激和恢复条件下.
- 进行了组织学 (HE,TUNEL) 和传输电子显微镜分析.
- 转录组分析是在组织和单核水平进行的.
主要成果:
- 热应激诱导了肌纤维细胞损伤,线粒体变化和自.
- 单核RNA测序确定了不同的细胞类型,包括肌核和肌肉干细胞 (MuSCs).
- 鉴定了热敏细胞群,特别是IIb型核细胞,以及MuSCs中与修复相关的基因表达.
结论:
- 该研究提供了迄今为止最大的热暴露骨肌肉的转录组数据集.
- 肌肉对热应激的反应在16小时的恢复后在组织层面上得到解决.
- 肌纤维,特别是IIb型肌核,被确定为最热敏的细胞类型,揭示了分子修复途径.
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