循环的 lncRNAs 评论 脑血管形表达特征 内皮细胞
Domenico Mordà1,2, Luigi Donato2,3, Simona Alibrandi2,3
1Department of Veterinary Sciences, Polo Universitario dell'Annunziata, Messina, Italy.
概括
这项研究确定了特定的长非编码RNA (lncRNA) 参与大脑动脉静脉形形 (bAVM) 和脑洞形形 (CCM). 这些lncRNAs可能为这些脑血管疾病提供新的诊断和治疗点.
科学领域:
- 血管生物学和遗传学
- 分子瘤学分子瘤学
- 神经科学是一个神经科学.
背景情况:
- 大脑动脉静脉形 (bAVM) 和脑洞形 (CCM) 是影响微血管的脑血管形.
- 它们的独特起源涉及受损的内皮分化 (bAVM) 和异常血管生成 (CCM).
- 长非编码RNAs (lncRNAs) 在竞争性内源RNA (ceRNA) 网络中的作用与各种疾病有关,但在脑血管表型中未得到充分研究.
研究的目的:
- 从bAVM和CCM患者的内皮细胞 (EC) 中识别差异表达的lncRNAs.
- 调查这些lncRNAs在bAVM和CCM的致病性中的潜在作用.
- 探索ncRNAs作为bAVM和CCM的潜在诊断标记和治疗点.
主要方法:
- 对bAVM和CCM活检的EC进行了RNA测序 (RNA-seq) 和丰富分析.
- 定量实时PCR (qRT-PCR) 用于验证EC和患者和对照的血液样本中的lncRNA表达.
- 生物信息分析确定了针对性的miRNA和与已识别的lncRNA相关的丰富途径.
主要成果:
- 五个lncRNAs (MIR497HG,RMST,SNHG6,SOX2-OT,ZSCAN16-AS1) 在bAVM EC和血液中过度表达.
- 在CCM EC中,FGD5-AS1和SNHG12被调高,而XIST,NORAD,SNHG29和LINC0066被调低.
- 对bAVM的丰富途径包括动脉静脉分化和光滑肌肉细胞功能;对于CCM,Rho-GTPase,Delta-Notch和VEGF信号是突出的.
结论:
- 特定的lncRNAs在bAVM和CCM中表达不同,这表明它们参与了不同的病原遗传机制.
- 这些lncRNAs可以作为ceRNAs发挥作用,调节与每个条件相关的miRNA目标.
- 这些发现突出了研究bAVM和CCM的发病,诊断和治疗的新领域.
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