微生物群的人性化驱动着猪的类似人类的代谢和免疫转录基因转移
Zhaoqi Zhang1,2,3,4, Yanan Xu1, Kun Pang5
1State Key Laboratory of Membrane Biology, Institute of Zoology Chinese Academy of Sciences Beijing China.
概括
移植到猪体内的人类肠道微生物创造了肠道微生物群人性化 (GMH) 猪. 转基因猪表现出类似人类的肠道细菌,代谢物和免疫细胞,推动了异种移植研究.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 代谢学 代谢学 代谢学
- 异种移植研究 异种移植研究
背景情况:
- 猪对于异种移植和生物医学研究具有价值.
- 肠道微生物群,免疫力和新陈代谢的跨物种差异阻碍了猪的利用.
- 人化猪微生物群对于翻译应用至关重要.
研究的目的:
- 通过移植人类便微生物群来建立肠道微生物群人性化 (GMH) 猪.
- 综合分析人类微生物群对猪生理学的影响.
- 评估转基因猪作为人类疾病和异种移植模型的潜力.
主要方法:
- 人类便微生物群移植到用抗生素治疗的猪身上.
- 使用元基因组学,准向代谢学和单细胞RNA测序 (scRNA-seq) 的综合分析.
- 系统评估微生物群组成,血清代谢物和免疫细胞概况.
主要成果:
- 转基因猪表现出类似人类的肠道微生物群组成,其中含有丰富的细菌体和枯竭的细菌体.
- 转基因猪的血清代谢概况部分趋于人类概况,具有改变的托代谢,胆酸和脂肪酸途径.
- scRNA-seq揭示了GMH猪中T细胞,单细胞和B细胞子集的显著重塑.
结论:
- 人体便微生物群移植有效地重塑了猪的系统代谢和免疫细胞特征.
- 转基因猪代表了一个强大的大型动物模型,用于研究宿主微生物群相互作用.
- 这种方法在异种移植和基于微生物组的治疗中推进了翻译应用.
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