相关实验视频
Updated: Feb 13, 2026

08:17
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
Published on: October 30, 2016
22.0K
基于拉曼流细胞计的单细胞物种分类,活细胞计数和益生菌产品的活力测试
Jia Zhang1,2,3, Jianmei Wang1,3, Pengfei Zhu1,2,4,5
1Single-Cell Center, Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Qingdao New Energy Shandong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao Shandong China.
概括
拉曼流细胞计 (RFC) 为评估益生菌质量提供了更快,更准确的方法. 这种先进的技术改善了活细胞产品的物种识别和生存能力测试.
科学领域:
- 微生物学 微生物学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 益生菌市场日益增长,需要有效的质量控制方法.
- 以前的显微镜成像和拉曼光谱 (miRS) 方法缺乏广泛应用的吞吐量.
- 单细胞拉曼光谱 (SCRS) 提供了高分辨率,但速度很慢.
研究的目的:
- 开发一种高通量拉曼流细胞计 (RFC) 方法来评估益生菌的质量.
- 提高物种和菌株识别的准确性和敏感性.
- 为了快速量化可活细胞和单细胞活力,而无需光标签.
主要方法:
- 实行拉曼流细胞计 (RFC) 系统用于细菌分析.
- 在高通量 (>1细胞/秒) 获得单细胞拉曼光谱 (SCRS).
- 利用指纹区域和D2O探测的RFC进行可行性和活力评估.
主要成果:
- 据RFC证明,通过率高于miRS的10倍.
- 显著提高了物种和菌株分类的准确性,即使在复杂的混合物中也是如此.
- 在检测低丰度成员方面获得了数量级更高的灵敏度.
- 在没有光标记的情况下,RFC准确量化了可活细胞和生命力,超过了基于PMA的方法.
结论:
- RFC是一个强大的,高吞吐量平台,用于快速准确地评估益生菌的质量.
- 该方法为活细胞产品分析提供了速度,灵敏度和丰富的信息.
- RFC提供了一种无标签的替代方法来评估细胞活力和活力.
相关概念视频
Flow Cytometry
16.5K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
In...
16.5K
Formation of Species
45.4K
Speciation describes the formation of one or more new species from one or sometimes multiple original species. The resulting species are discrete from the parent species, and barriers to reproduction will typically exist. There are two primary mechanisms, speciation with and without geographic isolation—allopatric and sympatric speciation, respectively.
45.4K
What is a Species?
50.6K
Overview
50.6K
Introduction to Vital Signs
8.7K
Vital signs are physiological measurements that help key into the status of the body's essential functions. These include body temperature, pulse rate, respiratory rate, and blood pressure, commonly abbreviated as T, P, R, and BP. Some healthcare settings also consider oxygen saturation (SpO2) and, in specific contexts, pain and level of consciousness as additional vital signs.
Vital signs help healthcare professionals assess an individual's well-being and detect any functional changes...
Vital signs help healthcare professionals assess an individual's well-being and detect any functional changes...
8.7K
Keystone Species
25.0K
Measures of species biodiversity, such as richness (i.e., the number of species present) and evenness (i.e., their relative abundance), describe an ecological community’s structure. Many factors affect community structure, including abiotic factors (e.g., sunlight and nutrients), disturbances (e.g., fire or flood), species interactions (e.g., predation or competition), and chance events (e.g., foreign species invasion). Certain species—such as keystone species—also play a...
25.0K
Guidelines For Measuring Vital Signs
2.9K
Following these guidelines can help nurses accurately measure vital signs, assess changes in patient conditions, and provide timely treatment when necessary. Adhering closely to the guidelines ensures the accuracy and reliability of the results.
Before taking a patient's vital signs, a nurse would consider and assess the patient's comfort level and ensure appropriate equipment is available.
Before taking a patient's vital signs, a nurse would consider and assess the patient's comfort level and ensure appropriate equipment is available.
2.9K

