在新生儿败血症和瘤性肠球炎中快速检测互α抑制蛋白
medRxiv : the preprint server for health sciences
|February 12, 2026
概括
互α抑制蛋白 (IAIP) 是关键的炎症生物标志物. 一种新的快速测试 (LFIA) 能够准确地检测出患有败血症或死性肠球炎 (NEC) 的婴儿IAIP水平下降,有助于早期诊断.
科学领域:
- 生物化学 生化学
- 新生儿医学 新生儿医学
- 生物标志物发现发现
背景情况:
- 互αα抑制蛋白 (IAIP) 作为血清蛋白酶抑制剂起作用,并且已被确立为炎症生物标志物.
- 在人类和动物研究中观察到IAIP水平在败血症期间下降.
- 目前IAIP测量的标准是酶相关免疫吸收试验 (ELISA).
研究的目的:
- 开发和验证用于IAIP检测的快速,护理点侧流免疫试验 (LFIA).
- 为了比较用LFIA和ELISA测量到的IAIP水平,用于患有败血症和/或死性肠球炎 (NEC) 的婴儿.
- 评估IAIP水平对新生儿败血症和NEC的诊断性能.
主要方法:
- 一个多中心,涉及毒症,NEC和对照的婴儿的横截面研究.
- 使用ELISA和新开发的LFIA分析了血液样本.
- 在急性事件出现时和随后的72小时内测量IAIP水平.
主要成果:
- 与对照组相比,患有败血症,NEC和培养负性败血症的婴儿的IAIP水平显著降低.
- 通过ELISA和LFIA进行的IAIP测量显示出高相关性 (R2 = 0.9326).
- 在LFIA中,新生儿败血症的灵敏度为80.0%,特异性为92.3%,新生儿败血症的灵敏度为84.6%,特异性为86.7%.
结论:
- 在新生儿败血症和NEC中,IAIP水平显著下降.
- 开发的LFIA是检测IAIP水平的可靠和快速方法,与ELISA有很好的相关性.
- LFIA显示出有希望的诊断潜力,用于早期识别新生儿的败血症和NEC.
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