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双模式的aptamer驱动生物传感平台,用于对SARS-CoV-2核囊蛋白的超敏感和突变弹性检测

Shu Zhou¹, Yuxi Xu¹, Huan Liao¹

¹State Key Laboratory of Chemo and Biosensing, College of Biology, Molecular Science and Biomedicine Laboratory, Hunan Research Center of the Basic Discipline for Cell Signaling, Hunan University, Changsha, Hunan 410082, China.

Genes & diseases

|February 12, 2026

概括

一个基于aptamer的新型生物传感平台MD ELAAA提供了对SARS-CoV-2的高度敏感和抗突变检测. 这种先进的诊断工具增强了对病毒及其变体的早期检测和监测.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断
纳米技术纳米技术

背景情况:

严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 的演变和高突变率挑战了现有的诊断方法.
当前分子诊断的局限性阻碍了全球有效的健康监测.
需要敏感和对突变有弹性的诊断平台至关重要.

研究的目的:

开发一种基于APTAMER的双模式SARS-CoV-2检测平台.
设计一种具有高灵敏度和对病毒突变的弹性生物传感系统.
加强早期诊断和高通量变种监测.

主要方法:

计算机辅助的X-aptamer通过指数式丰富 (SELEX) 确定了NP14 DNA的APTAMER.
分子对接,阿帕特默切割和突变发生阐明了NP14结合相互作用.
整合NP14到一个多色的动态光散射增强酶结合的aptamer-抗体测定 (MD ELAAA) 与Au/Ag纳米材料.

主要成果:

NP14体表现出高亲和度,双目标结合SARS-CoV和SARS-CoV-2核体蛋白质.
MD ELAAA的灵敏度为0.43 TCID50/ml,比标准方法提高了47倍.
该平台展示了高特异性,变体识别和双模式信号输出.

结论:

MD ELAAA平台可提供可靠的低丰富性SARS-CoV-2抗原的检测.
这种基于aptamer的生物传感器为下一代病毒检测和监测提供了强大的解决方案.
该系统的性能支持早期诊断和病毒演变的监测.

关键词:

亚普特美尔 (Aptamer) 是一种药物.适应剂-抗体三明治试验动态光散射是一种动态光散射.突变突变是一种突变.在SARS-CoV-2中,N蛋白质是N蛋白质.

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