在修复性和亲纤维性微环境中对透镜受伤的反应中对蛋白质甘氨酸表达的差异影响
Janice L Walker1,2, A Sue Menko1,2
1Department of Pathology and Genomic Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
概括
受伤的透镜上皮细胞在受伤的反应中表现出改变的蛋白质甘氨酸表达. 纤维化环境显著影响蛋白质甘氨酸的表达,影响细胞迁移和肌纤维细胞激活.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 镜片上皮细胞 (LEC) 通常在受伤后经历再生修复.
- 镜片内的免疫细胞在指导LEC迁移和伤口关闭方面发挥着至关重要的作用.
- 一个亲纤维的微环境可以诱导居住免疫细胞中的肌纤维细胞分化.
研究的目的:
- 为了比较LECs在修复性和亲纤维状况下的蛋白质甘氨酸表达变化.
- 研究微环境在调节LEC对受伤反应中的作用.
- 为了确定参与纤维化诱导的特定蛋白质糖.
主要方法:
- 活体透镜上皮层扩展培养模仿白内障手术.
- 无偏的RNA测序 (RNAseq) 分析蛋白质甘氨酸表达的分析.
- 在受伤后的三天内,在修复性和亲纤维性微环境中比较蛋白质甘氨酸样本.
主要成果:
- 修复性和亲纤维性环境都改变了与细胞迁移相关的蛋白质甘氨酸表达.
- 亲纤维的环境诱导了蛋白质甘氨酸表达中最显著的变化.
- 在纤维化中,参与TGF-β信号传递和原纤维化发生的特定的小型氨酸丰富蛋白质糖 (SLRPs) 被上调调节.
- 与机械传导和焦点粘附组合相关的细胞表面蛋白质甘在亲纤维的环境中被诱导.
结论:
- 微环境线索深深地影响受伤透镜表皮中的蛋白质甘氨酸表达.
- 纤维化微环境促进蛋白质甘氨酸的变化,促进肌纤维细胞激活和细胞外基质重塑.
- 了解这些蛋白质甘氨酸的动态对于开发预防眼部纤维化策略至关重要.
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