一个人类皮肤微生物的参考目录和平原成人的皮肤微生物景观
Yi Liu1, Zhiming Li2,3,4,5, Chao Zhang1
1Department of Dermatology, Shanghai Key Laboratory of Molecular Medical Mycology, Shanghai Changzheng Hospital Naval Medical University Shanghai China.
概括
这项研究创建了全面的人类皮肤微生物组基因组和蛋白质目录. 这些资源揭示了高原成年人的独特皮肤微生物特征以及影响皮肤细菌的因素.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 人类微生物组研究研究
背景情况:
- 人体皮肤微生物群的准确分类和功能分析需要强大的参考基因组.
- 现有的微生物基因组目录不足以进行全面的人体皮肤微生物组研究.
研究的目的:
- 建立综合的人体皮肤微生物组基因组 (HSMG) 和蛋白质 (HSMP) 目录.
- 用开发的目录来描述平原成年人人体皮肤微生物组的特征.
- 确定影响皮肤微生物组组成的因素.
主要方法:
- 整合了739个新测序的人类皮肤样本和2520个已发表的人类皮肤样本.
- 包括两个已发表的微生物基因组目录来构建HSMG和HSMP.
- 对HSMG和HSMP进行生物信息分析,用于物种识别和蛋白质表征.
- 基于皮肤类型和习惯的平原成年人皮肤微生物组特征的比较分析.
主要成果:
- 在HSMG中包括3547种 prokaryotic 物种,其中43.87%未被识别.
- HSMP含有超过3900万个非冗余蛋白,其中64.8%的特征不佳.
- 在高原成年人中,确定了明显的皮肤微生物组特征和生物地理特征.
- 在脂质和干燥皮肤微生物之间观察到显著差异,其中1784种 prokaryotes 变化.
- 发现宿主特征,护肤和日光照射显著影响皮肤微生物组.
结论:
- 开发的HSMG和HSMP为人类皮肤微生物组研究提供了宝贵的资源.
- 这项研究提供了在高原环境中皮肤微生物组的全面特征.
- 了解未培养的皮肤细菌的多样性对于微生物组功能分析至关重要.
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