相关实验视频
Updated: Feb 13, 2026

07:06
A Permanent Window for Investigating Cancer Metastasis to the Lung
Published on: July 1, 2021
5.6K
瘤外体L1细胞粘附分子促进肺癌的大脑转移
Dong Ha Kim1, Chae Won Lee1, Yun Jung Choi1
1Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
Research (Washington, D.C.)
|February 12, 2026
概括
肺癌外基因组通过通过L1CAM增加血脑屏障的透性来促进大脑转移. 一个结合的L1CAM/ITGB3生物标记面板显示了诊断大脑转移的潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 大脑转移 (BrM) 是肺癌的一个主要挑战,由血脑屏障 (BBB) 限制药物输送而恶化.
- 耐药肺癌细胞可能会分泌出影响BrM的外体.
- 识别外体介质对于理解和治疗BrM至关重要.
研究的目的:
- 调查BrM中耐药肺癌细胞外体的作用.
- 为了确定参与大脑转移的特定外体蛋白质.
- 评估已识别的BrM生物标志物的诊断潜力.
主要方法:
- 来自耐药肺癌细胞与父母肺癌细胞的外体的蛋白质组分析 (液体染色学-并联质谱学).
- 蛋白质与蛋白质相互作用和膜蛋白的生物信息分析.
- 对于候选蛋白质的验证,西式涂抹.
- 在体外和体外对外体L1CAM的功能性研究.
- 外体L1CAM和ITGB3作为生物标志物的临床验证.
主要成果:
- 耐药肺癌细胞衍生的外体显示出改变的蛋白质配置,其中744种蛋白质发生了显著的变化.
- 外体L1CAM和ITGB3被确定为与大脑特异性配体相互作用的关键候选者.
- 外体L1CAM通过增加BBB透性来增强大脑分布和BrM.
- 外体L1CAM破坏了内皮紧结完整性,涉及BBB细胞上的NCAM1.
- 外体L1CAM显示了BrM的诊断潜力 (AUC=0.80);一个L1CAM/ITGB3面板提高了准确性 (AUC=0.98).
结论:
- 外体L1CAM是肺癌脑转移的一个关键调节器.
- 外体L1CAM通过损害BBB完整性,有助于BrM.
- 外体L1CAM/ITGB3面板作为肺癌中BrM的非侵入性诊断生物标志物具有前途.
相关概念视频
Immunoglobulin-like Cell Adhesion Molecules
4.4K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
4.4K
Cell Adhesion Molecules - Types and Functions
9.7K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...
CAM Families
The Integrin family of proteins is primarily involved...
9.7K
Adhesion
44.6K
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
Capillary action is a result of water’s adhesive tendencies. When a narrow...
44.6K
Cancer Stem Cells and Tumor Maintenance
6.0K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.0K
Cell Adhesion in Plants
3.4K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
3.4K
The Eukaryotic Promoter Region
19.0K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
19.0K

