一个经过验证的LC-ESI-MS/MS试验,用于同时定量11种抗微生物药物的血量,以支持在重症儿童中个性化剂量
Yuan-Yuan Zhang1, Jie Wang1, Hong-Li Guo1
1Department of Pharmacy, Pharmaceutical Sciences Research Center, Children's Hospital of Nanjing Medical University, Nanjing, China.
Frontiers in molecular biosciences
|February 12, 2026
概括
一种新的LC-ESI-MS/MS方法准确地测量了重症患者血中的11种抗菌素. 这种快速测试支持治疗药物监测 (TDM),并优化了重症监护机构的抗菌剂量.
科学领域:
- 分析化学 分析化学
- 药理动力学 药理动力学
- 临床微生物学 临床微生物学
背景情况:
- 危急病患者表现出改变的药理动力学,需要精确的抗菌剂剂量.
- 目前用于抗微生物量化的分析方法面临技术限制,阻碍了有效的治疗药物监测 (TDM).
- 优化抗微生物剂量对于最大限度地提高疗效和最大限度地减少严重感染中的毒性至关重要.
研究的目的:
- 开发和验证一种快速,灵敏的LC-ESI-MS/MS方法,用于同时量化人体血中的11种抗微生物药物.
- 为临床TDM在重症监护机构提供可访问的分析方法.
- 支持在重症患者 (包括儿童) 中优化抗微生物剂量.
主要方法:
- 开发了一步蛋白质沉样本制备方法,使用甲醇与酸.
- 采用Kinetex C18柱,具有独特的梯度化程序,用于分析物分离.
- 使用LC-ESI-MS/MS与多重反应监测 (MRM) 在正离子化模式进行检测.
主要成果:
- 在8分钟内实现了11种抗微生物的基线分离,具有对称的峰值并且没有干扰.
- 证明了方法的稳定性,没有矩阵效应,具有很好的线性 (R2>0.99) 并符合验证标准.
- 成功地将验证的方法应用于儿科患者的血样本,证实了其临床效用.
结论:
- 经过验证的LC-ESI-MS/MS测定适用于临床TDM实施.
- 为优化患者治疗提供可靠和及时的抗生素度测量.
- 解决了对重症感染重症病房重症监护室可访问的分析方法的需求.
相关概念视频
Drug Dosing: Infants and Children
324
Pediatric patient dosages diverge from adults due to disparities in body surface area, total body water, and extracellular fluid per kilogram of body weight. The dosing regimen considers the variations in pharmacokinetics and pharmacology across distinct age groups, encompassing preterm newborns, infants, young children, older children, and adolescents. Calculation of pediatric patient doses is predicated on determining body surface area, which exhibits a superior correlation with the child's...
324
Reliability and Validity
14.1K
Reliability and validity are two important considerations that must be made with any type of data collection. Reliability refers to the ability to consistently produce a given result. In the context of psychological research, this would mean that any instruments or tools used to collect data do so in consistent, reproducible ways.
14.1K
Electrospray Ionization (ESI) Mass Spectrometry
2.4K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.4K
Antimicrobial Effectiveness
1.0K
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
1.0K
Self-Help Support Groups
363
Self-help support groups are voluntary, community-based organizations that provide a platform for individuals with shared concerns to exchange support, insights, and practical strategies for coping with life challenges. Typically led by group members or paraprofessionals, these groups form a cornerstone of mental health care, especially in reaching populations that are underserved by traditional healthcare systems.
Accessibility and Cost-Effectiveness
One of the primary strengths of self-help...
Accessibility and Cost-Effectiveness
One of the primary strengths of self-help...
363
Critical Region, Critical Values and Significance Level
13.4K
The critical region, critical value, and significance level are interdependent concepts crucial in hypothesis testing.
In hypothesis testing, a sample statistic is converted to a test statistic using z, t, or chi-square distribution. A critical region is an area under the curve in probability distributions demarcated by the critical value. When the test statistic falls in this region, it suggests that the null hypothesis must be rejected. As this region contains all those values of the...
In hypothesis testing, a sample statistic is converted to a test statistic using z, t, or chi-square distribution. A critical region is an area under the curve in probability distributions demarcated by the critical value. When the test statistic falls in this region, it suggests that the null hypothesis must be rejected. As this region contains all those values of the...
13.4K


