相关实验视频

Updated: Feb 13, 2026

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
11:22

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins

Published on: October 29, 2013

43.6K

一个单步方法,有效净化功能Cas9蛋白质

Xinzhi Duan1,2, Zhengrong Zhou2, Aihua Mao1,2

  • 1Biology Department, College of Sciences, Shantou University, Shantou, China.

Bio-protocol
|February 12, 2026
PubMed
概括

我们开发了一种简化的,单步CRISPR/Cas9净化方法,使用大肠杆菌中的全域化系统. 这种方法为基因组编辑应用提供了高纯度,功能性的Cas9蛋白.

关键词:
亲和色谱法是一种亲和色谱法.基因编辑 基因编辑蛋白质净化 蛋白质的净化这就是SpCas9的意义.乌比奎丁 - 融合 - 乌比奎丁

更多相关视频

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization07:08

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

14.4K
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

12.0K

相关概念视频

Structural Protein Function01:56

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.0K
Structural Protein Function01:56

Structural Protein Function

3.3K
Mechanical Protein Functions01:58

Mechanical Protein Functions

Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
5.7K
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.5K
Mechanical Protein Function01:58

Mechanical Protein Function

2.5K
Assessment of blood pressure in brachial artery(one-step method)01:15

Assessment of blood pressure in brachial artery(one-step method)

This procedural guide systematically measures blood pressure using an oscillometric digital sphygmomanometer, emphasizing accuracy, patient safety, and comfort.
Prepare for the Procedure:
1.2K