在乳酸细菌中抑制基质的机械双通道建模,以增强发酵控制
Guoxi Zheng1,2, Junwen Mao1
1College of Science Huzhou University Huzhou Zhejiang China.
Quantitative biology (Beijing, China)
|February 12, 2026
概括
乳酸细菌 (LAB) 发酵中的基质抑制阻碍了有效的乳酸生产. 一个新的双通道模型解释了这一点,揭示了在高基质水平的长期滞后阶段,并建议进行预培养治疗以改善生物过程.
科学领域:
- 微生物学 微生物学
- 生物化学工程 生物化学工程
- 系统生物学 系统生物学
背景情况:
- 乳酸细菌 (LAB) 发酵中的基质抑制降低了高基质度的细胞生长和乳酸生产.
- 基质抑制背后的分子和生理机制尚未完全理解.
- 现有的运动模型很难完全捕捉基质抑制的复杂动态.
研究的目的:
- 提出一种机械的双通道模型,将分子调节整合到微生物生长和基质同化中.
- 为了阐明不同初始基质度的LAB发酵中基质抑制的动态.
- 确定减轻基质抑制和优化生物过程的策略.
主要方法:
- 开发一种机械式的双通道模型,包括基质响应分子调节.
- 建模分析以捕捉全球增长动态 (滞后,指数,静止阶段).
- 模型的验证使用LAB批发发酵的已发表的实验数据 (例如,乳糖上的Lactobacillus bulgaricus,Lactobacillus casei,乳糖上的Lactiplantibacillus plantarum).
主要成果:
- 该模型准确地预测了在高初始基质度下延长的滞后阶段.
- 该模型证明了在不同的LAB菌株和基板中具有普遍性.
- 模型模拟表明,培养前治疗可以减轻基质抑制效应.
- 根据注射器尺寸变化,可以预测最佳的微生物生长动态.
结论:
- 拟议的双通道模型提供了对LAB发酵中的基质抑制的机制性理解.
- 预培养处理成为一种可行的策略,以提高高基板环境中的发酵效率.
- 建模方法为优化生物工艺工程中的基质利用提供了洞察力.
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