基于DCIP和Eu3+-calcein的双模式生物传感器用于性酸酶检测
Bingyu Fu1,2,3, Yanru Shao1, Zhixiong Zheng1
1College of Life Science, Yangtze University, Jingzhou, Hubei, 434025, China. xuqi@yangtzeu.edu.cn.
Analytical methods : advancing methods and applications
|February 12, 2026
概括
这项研究引入了一种双模式生物传感器,用于检测性酸酶 (ALP). 生物传感器利用色度和光信号,在样本中提供敏感和选择性检测ALP.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 酸酶 (ALP) 是一个关键的酶,参与各种生理和病理过程.
- 精确检测ALP对于诊断肝脏疾病和骨疾病等疾病至关重要.
- 现有的生物传感器往往缺乏灵敏度,选择性或双模式检测能力.
研究的目的:
- 开发一种简单而灵敏的双模式生物传感器,用于性酸酶 (ALP) 检测.
- 为了提高检测准确度,利用色度和光信号.
- 在复杂的生物矩阵中验证生物传感器的性能,例如血清样本.
主要方法:
- 设计了一种双模式生物传感器,采用2,6-二二 (DCIP) 色度和素光.
- 使用L-酸-2- (AAP) 作为基质,由ALP去化成酸 (AA) 和离子.
- 利用AA的降解性质去色DCIP和酸离子的化能力来恢复Eu3+-素光.
主要成果:
- 测色模式:线性检测范围为1.56-50U L-1 ,检测极限 (LOD) 为0.27U L-1 .
- 光模式:线性检测范围为0.78-25 U L-1 ,LOD为0.23 U L-1 .
- 在血清样本中表现出良好的ALP选择性和令人满意的恢复率.
结论:
- 开发的双模式生物传感器为ALP检测提供了一种简单,灵敏和选择性的方法.
- 综合色度和光方法提高了检测可靠性.
- 这种生物传感器显示出临床诊断和生物化学分析的潜力.
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