在生物成像应用中,用于增强细胞吸收的Eu(III) 化酸纳米颗粒的合成后酸盐修饰
Thoudam Chanchan Devi1, Pritam Das2,3, Souma Ghosh2,3
1Department of Chemistry, National Institute of Technology Manipur, Langol, 795004, India. davidthiyam@nitmanipur.ac.in.
Dalton transactions (Cambridge, England : 2003)
|February 12, 2026
概括
酸盐修饰增强了用于生物成像应用的欧洲化酸纳米颗粒 (Eu:HAp NPs) 的细胞吸收和生物相容性. 这种表面功能化可以提高纳米粒子的稳定性,而不会影响发光.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
背景情况:
- 生物仿真纳米粒子 (NP) 显示出生物医学应用的前景,这是由于其高效的细胞吸收.
- 酸纳米粒子 (HAp NPs) 是有趣的,并且用欧 (Eu(III)) 进行注可以赋予有用的光发光特性.
- 表面修饰对于优化生物系统中的NP性能至关重要.
研究的目的:
- 研究酸盐作为Eu(III) 化酸纳米颗粒 (Eu:HAp NPs) 的合成后表面修饰剂.
- 评估酸盐修饰对Eu:HAp的结构,体和光发光特性的影响.
- 为了评估裸体和酸盐修饰的Eu:HAp NP的生物相容性和细胞吸收,用于生物成像应用.
主要方法:
- 合成Eu:HApNP和随后的表面修饰用酸盐.
- 使用X射线衍射 (XRD),里埃变换红外光谱 (FTIR),场发射扫描电子显微镜 (FESEM),传输电子显微镜 (TEM) 和动态光散射 (DLS) 的表征.
- 使用HEK-293和MDA-MB-231细胞系评估生物相容性,并通过共聚焦成像和光激活细胞分类 (FACS) 评估细胞吸收.
主要成果:
- 酸盐的修饰是通过化学吸收发生的,产生负电荷的碳酸盐位,并改善了合体稳定性,而不会改变Eu:HAp NP的核心结构或光发光.
- 裸体和酸盐修饰的Eu:HApNP都没有对测试的细胞系表现出显著的毒性.
- 与裸体NP相比,用酸盐修饰的Eu:HApNP表现出增强的细胞吸收和更强的红色发光.
结论:
- 酸盐修饰是一种有效的策略,可以增强Eu:HApNP的生物相容性和细胞吸收.
- 修改后的NP非常适合用于生物成像应用,原因是改善了细胞相互作用和固有的发光.
- 这种表面功能化方法有可能用于各种生物医学应用的目标连接体.
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