基于SERS的通用AST:在物种识别之前,血液培养细菌的快速治疗指南
Yin-Yi Han1,2, Yu-Tsung Huang3, Dai-Feng Li3
1Department of Anesthesiology, National Taiwan University College of Medicine, Taipei 100225, Taiwan.
Analytical chemistry
|February 12, 2026
概括
一个新的物种独立平台SERS-Uni-AST在5小时内快速确定细菌对抗生素的敏感性. 这一进步有助于及时治疗血液感染,改善患者的治疗结果.
科学领域:
- 生物医学工程 生物医学工程
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 血流感染 (BSI) 的死亡率很高.
- 目前的抗微生物敏感性测试 (AST) 方法通常需要细菌物种的识别,延迟治疗.
- 快速的AST对于有效的BSI管理和抗菌药物管理至关重要.
研究的目的:
- 开发一种独立于物种的平台,用于快速抗微生物敏感性测试 (AST).
- 为了实现更快,更适应性AST用于血液感染,包括罕见或未知的物种.
- 支持早期,受敏感性引导的抗微生物疗法.
主要方法:
- 开发了SERS-Uni-AST,这是一个使用表面增强拉曼散射 (SERS) 的物种独立平台.
- 使用无标签检测来监测细菌对抗生素的代谢反应.
- 在43种细菌物种和7种抗生素中对191个临床隔离物验证了平台.
主要成果:
- 在5小时内,SERS-Uni-AST与标准AST达成92%的分类一致.
- 在包括ESKAPE病原体在内的格拉姆阳性和格拉姆阴性细菌中表现出强大的性能.
- 使用预定义的,独立于物种的决策值来获得可靠的结果.
结论:
- SERS-Uni-AST为AST提供了一个快速的,独立于物种的解决方案,大大缩短了周转时间.
- 该平台简化了工作流程,并将应用范围扩展到已知的细菌物种之外.
- 能够及时,易感性导向治疗,与生存败血症运动和世卫组织抗菌药物管理目标保持一致.
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