在实验性结肠炎中,aucubin通过微生物群-SCFA-GPR41/GPR43轴恢复肠粘膜免疫和屏障完整性
Zhen Zhang1,2, Jingjing Yang1,2, Xiaohua Chen1,2
1Department of Gastrointestinal Surgery, First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, China.
概括
奥库 (Aucubin,AU) 通过恢复肠道微生物群平衡和免疫平衡来安全地治疗小鼠的大肠炎. 它通过短链脂肪酸 (SCFA) 和GPR41/43通路增强肠道屏障功能,提供了一个有前途的治疗方法.
科学领域:
- 胃肠道学和免疫学
- 微生物组研究 微生物组研究
- 自然产品治疗药物 自然产品治疗药物
背景情况:
- 炎症性肠病 (IBD) 涉及免疫功能障碍和肠道微生物群失衡.
- 奥库 (Aucubin,AU) 是一种天然的虹膜糖化物,具有抗炎性质,但其在结肠炎中的作用尚不清楚.
研究的目的:
- 研究Aucubin (AU) 对小鼠中硫酸 (DSS) 诱导的大肠炎的治疗作用.
- 阐明AU功效背后的机制,包括免疫细胞平衡,肠道屏障完整性,肠道微生物群调节以及SCFA及其受体的作用.
主要方法:
- 在接受AU治疗的小鼠中评估了DSS诱导的大肠炎,评估了疾病严重程度,免疫细胞种群 (Tregs/Th17),肠道屏障标记物 (MUC2,紧密结节) 和炎症性细胞因子.
- 使用16S rRNA测序分析了肠道微生物组成,并测量了便短链脂肪酸 (SCFA).
- 研究了使用抗生素治疗小鼠和便微生物种移植 (FMT) 的微生物群依赖性影响,并使用受体对抗剂评估了GPR41/43的作用.
主要成果:
- AU治疗显著改善了DSS诱导的大肠炎,通过重新平衡Treg/Th17细胞来恢复免疫平衡.
- 通过对MUC2和紧密结合蛋白进行上调,AU增强了肠道屏障功能.
- AU调节了肠道微生物群,增加了SCFA生产的种群和便中的SCFA水平. 它的治疗效果依赖于微生物群,部分由SCFAs-GPR41/43轴介导.
结论:
- 奥库 (Aucubin,AU) 显示出对小鼠大肠炎的安全有效治疗潜力.
- 通过重新平衡肠道微生物群,增强SCFAs-GPR41/43信号通路,促进免疫调节和修复肠道屏障来缓解大肠炎.
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