评估肠表皮屏障完整性的工具和先进成像技术:系统性审查
Sophie Vieujean1, Raja Atreya2, Andrea Buda3
1Department of Gastroenterology, Cliniques Universitaires Saint-Luc, Brussels, Belgium.
American journal of physiology. Gastrointestinal and liver physiology
|February 12, 2026
概括
评估肠上皮质屏障的完整性对于诊断肠道疾病至关重要. 目前的方法在准确性和标准化方面有所不同,这突显了需要改进的诊断工具的需要.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 生物医学工程 生物医学工程
背景情况:
- 肠上皮形成了一个关键的屏障,调节营养吸收和抗原进入.
- 屏障功能障碍导致炎症和各种胃肠道和全身疾病.
- 早期发现上皮质变化对于治疗干预至关重要.
研究的目的:
- 系统地审查目前评估肠道上皮质屏障变化的方法.
- 评估体内和体外评估技术的优点和局限性.
主要方法:
- 非侵入性和侵入性体内方法的概述 (例如,尿道探针,生物标志物,共聚焦激光内分显微镜).
- 对ex vivo/in vitro方法的审查 (例如,跨体电阻,阻抗光谱,有机电化学晶体管).
主要成果:
- 非侵入性方法缺乏标准化和特异性.
- 侵入性内镜技术提供实时可视化,但是昂贵的研究工具.
- 像TEER这样的体外试验方法是标准的,新技术显示出有前途.
- 生物标志物和微生物转移指标需要进一步验证.
结论:
- 在定义障碍分解,诊断工具和验证方面存在重大差距.
- 方法的异质性和未经验证的值阻碍了临床应用.
- 需要进一步的研究来开发标准化,验证的诊断方法来检测肠道屏障的功能.
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