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在消防员的尿液中检测和描述miRNA的变化
Patrick C Sachs1, Julie S Bjerring1, James Blando2
1School of Medical Diagnostic & Translational Sciences, Old Dominion University, Norfolk, VA, USA.
概括
消防员接触有毒化学物质会增加癌症的风险. 尿液微RNA (miRNA) 显示为评估消防员健康风险和对火灾事件的生理反应的生物标志物.
科学领域:
- 环境健康 环境健康
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 消防员在灭火过程中面临着大量的有毒化学物质暴露.
- 流行病学研究表明,消防员患各种癌症的风险较高.
- 非侵入性生物标志物对于有效的风险管理和健康监测至关重要.
研究的目的:
- 为了识别与消防接触相关的尿路微RNA (miRNA) 概况.
- 研究miRNAs作为消防员生理反应的生物标志物的潜力.
- 为在消防员的健康风险评估中使用尿路miRNA建立一个框架.
主要方法:
- 从消防员 (暴露前和暴露后) 和健康对照者收集尿液样本.
- 使用Affymetrix GeneCHIP miRNA 4.0数组进行了微RNA (miRNA) 分析.
- 生物信息分析 (DAVID) 用于识别基因标和相关途径.
主要成果:
- 在消防员和对照者中,23个miRNAs显著上调,25个miRNAs显著下调.
- 纵向分析显示,20个miRNAs在火灾暴露后立即上调.
- 已识别的miRNA及其基因标与癌症和炎症性疾病有关.
结论:
- 尿液miRNA资料可以表明对灭火的生理反应.
- 特定的miRNA显示出作为消防员健康风险评估的非侵入性生物标志物的潜力.
- 这些发现支持使用miRNA分析来监测消防暴露对健康的影响.
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