开发基于RPA-LFD的核桃炭酸的快速视觉检测方法
Yue Liu1, Weishan Zhang2, Jing Cao3
1Beijing Forestry University College of Forestry, Beijing Forestry University, 35 East Qinghua Road, Haidian District, Beijing, China, 100083; liuyue000@bjfu.edu.cn.
Plant disease
|February 12, 2026
概括
一种新的重组酶聚合酶放大与侧流滴杆 (RPA-LFD) 试验相结合,可以快速检测多种导致核桃炭的Colletotrichum物种. 这种广泛的方法克服了单个病原体分析的局限性,改善了疾病管理.
科学领域:
- 植物病理学 植物病理学
- 分子诊断学 分子诊断
- 菌类学 菌类学是指菌类学.
背景情况:
- 由Collettotrichum真菌引起的核桃炭,很难使用当前的单个病原体测定来准确检测.
- 现场复杂的Colletotrichum种群往往会导致假阴性,阻碍有效的疾病管理.
研究的目的:
- 开发一种快速,宽频谱的检测方法,用于负责核桃炭菌的科莱托里丘姆物种.
- 克服现有测试在识别各种真菌种群的局限性.
主要方法:
- 开发一种复合酶聚合酶放大与侧向流量测量棒 (RPA-LFD) 试验相结合的方法.
- 针对保存的内部转录间隔器 (ITS) 区域,使用专门设计的启动器和探针.
- 系统选,以选择最佳的原料和探针集,以特定性和灵敏性.
主要成果:
- 优化的RPA-LFD测定在39°C时在10分钟内检测到多种Colletotrichum物种.
- 该试验显示高特异性,没有与其他与核桃相关的真菌的交叉反应.
- 敏感度值因物种而异,有些物种检测到10 pg/μL,有些物种检测到100 pg/μL.
结论:
- RPA-LFD系统提供了一种简单,敏感和特定的方法来检测Colletotrichum物种.
- 视觉结果的解释和快速的周转时间使其适合于现场应用.
- 这项技术有很大的潜力,可以改善核桃炭病管理策略.
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