Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Induced-fit Model01:13

Induced-fit Model

89.7K
Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
89.7K
Inclusive Fitness00:57

Inclusive Fitness

42.3K
Most altruistic behavior—in which one animal helps another at a cost to themselves—occurs between relatives. Scientists think these altruistic behaviors evolved because they increase the inclusive fitness of the animal providing help.
42.3K
Goodness-of-Fit Test01:16

Goodness-of-Fit Test

9.3K
The goodness-of-fit test is a type of hypothesis test which determines whether the data "fits" a particular distribution. For example, one may suspect that some anonymous data may fit a binomial distribution. A chi-square test (meaning the distribution for the hypothesis test is chi-square) can be used to determine if there is a fit. The null and alternative hypotheses may be written in sentences or stated as equations or inequalities. The test statistic for a goodness-of-fit test is given as...
9.3K
Expected Frequencies in Goodness-of-Fit Tests01:19

Expected Frequencies in Goodness-of-Fit Tests

8.7K
A goodness-of-fit test is conducted to determine whether the observed frequency values are statistically similar to the frequencies expected for the dataset. Suppose the expected frequencies for a dataset are equal such as when predicting the frequency of any number appearing when casting a die. In that case, the expected frequency is the ratio of the total number of observations (n)  to the number of categories (k).
8.7K
Statistical Methods to Analyze Parametric Data: Student t-Test and Goodness-of-Fit Test01:09

Statistical Methods to Analyze Parametric Data: Student t-Test and Goodness-of-Fit Test

7.0K
In parametric statistics, two fundamental tests stand out for their utility and wide application: the Student's t-test and goodness-of-fit tests. These tests provide researchers with a robust method for drawing insights from data, testing hypotheses, and making informed decisions based on their findings.
The Student's t-test is a statistical test that examines if there is a statistically significant difference between the means of two groups. This test is instrumental when dealing with...
7.0K
What are Cells?01:07

What are Cells?

205.3K
Cells are the smallest and basic units of life, whether it is a single cell that forms the entire organism, e.g., in a bacterium or trillions of them, e.g., in humans. No matter what organism a cell is a part of, they share specific characteristics.
Basic Characteristics of Cells
A living cell has a plasma membrane, a bilayer of lipids that separates the aqueous solution inside the cell called the cytoplasm from the outside environment.
Furthermore, a living cell possesses genetic information...
205.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

TDP-43 dysfunction compromises UPF1-dependent mRNA metabolism in ALS.

Neuron·2025
Same author

Gene regulation through exon junction complex modularity.

Nature structural & molecular biology·2025
Same author

FMRP drives mRNP targets into translationally silenced complexes.

Molecular cell·2025
Same author

Corrigendum: Exploring the therapeutic potential of modulating nonsense-mediated mRNA decay.

RNA (New York, N.Y.)·2025
Same author

Exploring the therapeutic potential of modulating nonsense-mediated mRNA decay.

RNA (New York, N.Y.)·2024
Same author

Nuclear mRNA decay: regulatory networks that control gene expression.

Nature reviews. Genetics·2024
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
查看所有相关文章

相关实验视频

Updated: Feb 14, 2026

A Quantitative Fitness Analysis Workflow
11:39

A Quantitative Fitness Analysis Workflow

Published on: August 13, 2012

15.0K

保持细胞的健康 保持细胞的健康

Xavier Rambout1,2, Lynne E Maquat1,2

  • 1Department of Biochemistry & Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA.

Science (New York, N.Y.)
|February 12, 2026
PubMed
概括
此摘要是机器生成的。

异常的细胞质信使RNA (mRNA) 片段与核RNAs结合. 这种相互作用增强了基因转录的过程,影响了基因表达调节.

更多相关视频

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
08:53

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Published on: August 31, 2016

16.0K
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

16.0K

相关实验视频

Last Updated: Feb 14, 2026

A Quantitative Fitness Analysis Workflow
11:39

A Quantitative Fitness Analysis Workflow

Published on: August 13, 2012

15.0K
Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
08:53

Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Published on: August 31, 2016

16.0K
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

16.0K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 在RNA生物学,RNA生物学.

背景情况:

  • 细胞质mRNA通常在核外发挥作用.
  • 核RNA在核中的基因调节中发挥作用.
  • 异常RNA物种可以从各种细胞过程中产生.

研究的目的:

  • 为了研究异常细胞质mRNA片段的功能作用.
  • 为了确定细胞质mRNA碎片和核RNA之间的相互作用.
  • 为了阐明这种相互作用对基因转录的影响.

主要方法:

  • 利用RNA测序来识别异常的细胞质mRNA片段.
  • 用于检测RNA-RNA相互作用的交叉链接免疫沉降 (CLIP) 试验.
  • 进行定量实时PCR (qRT-PCR) 来测量转录水平的变化.

主要成果:

  • 鉴定出异常细胞质mRNA的特定碎片.
  • 证明了这些mRNA片段与核RNAs的直接结合.
  • 在这种结合事件上观察到目标基因转录的显著增加.

结论:

  • 异常的细胞质mRNA片段可以转移到核中.
  • 这些片段通过与核RNA相互作用,积极参与基因调节.
  • 这种新的机制突出显示了一种新的转录控制层.