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集成的双模式生物传感器用于抗菌素敏感性测试和抗生素处方指南
Lin-Yan Lu1,2, Yi-Ming Zheng1,2, Jie-Wei Huang3
1Quanzhou Clinical Medication Management Quality Control Center, Department of Pharmacy, Affiliated Quanzhou First Hospital of Fujian Medical University, Quanzhou 362000, China.
一个新的双模式生物传感器通过测量细菌催化酶活性来准确检测抗菌耐药性 (AMR). 这种快速抗微生物敏感性测试 (AST) 方法提高了指导抗生素处方的诊断准确性.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 微生物学 微生物学
背景情况:
- 抗菌素耐药性 (AMR) 构成了全球健康的重大威胁.
- 快速的抗微生物敏感性测试 (AST) 对于有效的诊断和治疗至关重要.
- 现有的光和色度传感器可以产生错误的信号.
研究的目的:
- 通过克服单模传感器的局限性来开发精确可靠的AST方法.
- 通过融合双模式检测功能来提高诊断准确性.
- 创建一个生物传感器平台,用于指导抗生素处方的临床决策.
主要方法:
- 开发一种双模式的集成光/色度生物传感器.
- 使用牛血清蛋白修饰金纳米集群 (BSA-AuNCs) 作为双功能传感器.
- 评估抗生素暴露后的细菌催化酶活性,监测过氧化 (H2O2) 反应.
主要成果:
- 集成的双模式生物传感器准确地确定了对大肠杆菌,肺炎和黄金杆菌的抗生素敏感性.
- 实现了高诊断性能,曲线下的面积 (AUC) 值在0.9881到1.0之间.
- 与单模检测方法相比,证明了更好的准确性和可靠性.
结论:
- 开发的双信号读出生物传感器为AST提供了一个精确可靠的平台.
- 这种方法有效地解决了对抗AMR的诊断准确度提高的需要.
- 生物传感器有可能指导临床抗生素处方,优化患者护理.
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