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一个由酸还原酶触发的NIR光探针用于选择性可视化在 ортотоп性乳腺癌
Xiaoyu Wan1, Sisi Wang2, Pinyi Ma3
1Department of Breast Surgery, The Second Hospital of Jilin University, Changchun 130041, China.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|February 12, 2026
概括
一个新的近红外 (NIR) 光探测器DHM-NO2有效地可视化了乳腺瘤中缩酶 (NTR) 的活性. 这种探针为瘤的攻击性和治疗反应提供了改进的成像,克服了现有方法的局限性.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 化学生物学 化学生物学
- 分子瘤学分子瘤学
背景情况:
- 缩酶 (NTR) 在缺氧性乳腺瘤代谢中至关重要,表明了攻击性和治疗结果.
- 现有的NTR可视化探测器具有有限的光物理特性,阻碍了准确的成像.
研究的目的:
- 开发一种新的近红外 (NIR) 可激活的光探针,用于可视化乳腺癌中还原酶 (NTR) 活性.
- 为了克服当前探测器中短发射波长和小斯托克斯移动的局限性.
主要方法:
- 通过将DHM-OH光体与酸识别单元合而构建DHM-NO2.
- 在实验室使用乳腺癌细胞 (MCF-7) 和正常细胞 (MCF-10A) 评估探针性能.
- 评估了在 ортотоп性乳腺癌小鼠模型中的探针激活和瘤定位.
主要成果:
- DHM-NO2表现出微不足道的背景光和快速的NTR介导激活,释放DHM-OH用于NIR发射 (880nm).
- 探测器显示出高灵敏度 (25.6 pg/mL检测极限),选择性,以及对ROS/RNS干扰的抗性.
- 成功分化癌细胞,响应代谢调节,并在体内产生瘤局部化的光,瘤与正常的比例高.
结论:
- DHM-NO2是一种敏感和选择性的NIR-I光探针,用于监测乳腺癌中NTR活性.
- 探针的设计克服了以前的局限性,使瘤代谢和治疗反应的有效成像成为可能.
- DHM-NO2与标准的光仪器相容,方便临床翻译.
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