优化被暴露时间和被动采样器的材料选择,以改善废水监测便污染
Julius Look1, Urda Düker2, Regina Nogueira2
1Ostfalia University of Applied Sciences, Faculty Civil and Environmental Engineering, Center for Hydrosystems and Health, Germany.
被动采样器有效地检测废水中的细菌和耐药基因,提供近实时的公共卫生数据. 在基于废水的流行病学中,玻璃珠在沉积物提取和可重复性方面表现最好.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生监督是对公共卫生的监督.
- 分子诊断学 分子诊断学
背景情况:
- 被动采样证明了对实时SARS-CoV-2监测的有效性.
- 它们用于检测细菌和耐药基因的应用需要进一步开发和测试.
研究的目的:
- 分析各种被动采样器对微生物参数的适用性.
- 为了确定不同类型的被动采样器的最佳暴露时间.
- 评估基于废水的流行病学 (WBE) 的被动样本.
主要方法:
- 测试了七种被动采样机类型 (聚乙烯条纹,钢羊毛海绵,玻璃珠,鱼雷,笔,Q-Tip,过膜).
- 使用滴滴数字PCR (ddPCR) 分析了微生物参数,包括总细菌,肠球菌,甲基四环素和1类整体素.
- 开发了一种新的规范化方法,用于比较沉积物,生物膜和废水样本.
主要成果:
- 所有测试的被动样本检测到细菌和耐药基因在各种暴露时间 (2h至48h).
- 被动样本提供了与复合废水样本相比的正常化微生物比率.
- 在不同的被动采样器类型 (生物膜,沉积物,材料) 中,最佳暴露时间有所不同.
- 玻璃珠在沉积物提取中表现出最好的处理力和可重复性.
结论:
- 被动采样器是基于废水的流行病学可行的工具,可以检测细菌和耐药基因.
- 选择被动采样器材料和类型会影响最佳采样时间.
- 一种新的规范化方法使WBE中不同样本类型的直接比较更容易.
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