猪USP18通过调节I型干扰素信号通路来抑制日本脑炎病毒的复制
Haixin Shi1, Xiangchen Li1, Xiaolong Zhou1
1Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China.
Veterinary microbiology
|February 12, 2026
概括
猪USP18 (Ubiquitin特异性酶18) 通过增强I型干扰素信号传递来抑制日本脑炎病毒 (JEV) 复制. 这项研究揭示了USP18的价值.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 乌比基特异性酶18 (USP18) 对于先天免疫和抗病毒反应至关重要.
- 目前尚不清楚USP18在日本脑炎病毒 (JEV) 复制中的作用.
研究的目的:
- 为了研究猪USP18在JEV复制中的功能.
- 阐明USP18对JEV的抗病毒活性的潜在分子机制.
主要方法:
- 定量实时PCR (qRT-PCR) 和西式涂抹来分析USP18表达.
- 过度表达和siRNA介导的USP18.的淘汰.
- AlphaFold3预测,共免疫沉降 (Co-IP) 和共聚焦免疫光试验用于研究蛋白质相互作用.
- 对I型干扰素 (IFN-I) 信号通路基因表达的分析.
主要成果:
- JEV感染显著提高了猪USP18表达的调节.
- USP18的过度表达抑制了JEV的复制,而Knockdown则增强了它.
- USP18与IRF3和MAVS相互作用,促进IRF3的酸化,并增强IFN-I信号传输.
- USP18积极调节IFN-I介导的针对JEV的抗病毒反应.
结论:
- 猪USP18通过积极调节IFN-I抗病毒通路来抑制JEV复制.
- USP18与IRF3和MAVS的相互作用对其抗病毒功能至关重要.
- USP18为JEV耐药性繁殖和猪的抗病毒疗法提供了潜在的分子标.
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