流细胞测量参数用于预测MALDI-TOF MS在尿液中成功识别gram阴性菌根的预测
María Jesús Castaño1, Ignacio Torres2, Katherine Mishell Villarruel1
1Microbiology Service, Clinic University Hospital, Valencia, Spain.
Diagnostic microbiology and infectious disease
|February 12, 2026
概括
矩阵辅助激光脱吸电离-飞行质谱时间 (MALDI-TOF MS) 可以快速识别尿路感染 (UTI) 病原体. 流细胞计有助于选择 MALDI-TOF MS 的样本,提高效率和临床决策.
科学领域:
- 临床微生物学 临床微生物学
- 诊断技术 诊断技术的使用
- 传染性疾病 传染性疾病
背景情况:
- 尿路感染 (UTI) 需要快速诊断才能获得有效的抗菌疗法.
- 传统的培养方法可能会耗费大量时间.
- 矩阵辅助激光消耗离子化-飞行质谱的时间 (MALDI-TOF MS) 提供更快的微生物识别.
- 确定在尿路感染诊断中选择样本的最佳门对于效率至关重要.
研究的目的:
- 评估流细胞计与MALDI-TOF MS一起用于尿路感染诊断的实用性.
- 用流式细胞计量来确定 MALDI-TOF MS 处理的最佳细菌负荷值.
- 评估MALDI-TOF MS与传统培养方法之间的一致性.
主要方法:
- 分析了来自怀疑患有尿路感染的患者的652个尿样.
- 用流式细胞计量检测细菌负载 (细菌/μL) 和格拉姆染色.
- 使用MALDI-TOF MS和常规培养物进行微生物识别.
- 执行了接收器操作特征 (ROC) 分析,以确定最佳切断值.
主要成果:
- 在 MALDI-TOF MS 和培养之间,总体一致性很高 (96.5%).
- 确定了15606种细菌/μL作为MALDI-TOFMS处理的最佳流细胞计切断值 (灵敏度:83.3%,特异性:50%).
- MALDI-TOF MS在比传统的≥105 CFU/mL值更低的细菌负载下有效.
- 侧流免疫染色学 (LFIC) 测定有助于检测抗性机制.
结论:
- 流细胞计是有效的选择适当的样本,用于MALDI-TOFMS在尿路感染诊断.
- 已建立的流细胞测量值通过使MALDI-TOF MS在较低的细菌度下使用,从而提高了资源效率.
- 流细胞计和MALDI-TOF MS的联合使用可以提高尿路感染诊断和管理的速度和效率.
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