一个dCas9集成的iLight9O系统能够动态调节Saccharomyces cerevisiae中增强的patchoulol生物合成
Li Li1, Yasen Wang1, Buqing Wang1
1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Bioresource technology
|February 12, 2026
概括
一个新的光遗传工具,iLight9O,可以精确控制酵母中的基因表达. 这种系统通过动态下调竞争的代谢途径来增强帕丘产量,显著提高产量.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 视觉遗传学 视觉遗传学
背景情况:
- 光遗传系统通过光来调节生物过程.
- 现有的酵母光遗传工具用于基因抑制,通常需要插入目标部位.
- 精确调节内源酵母基因表达仍然是一个挑战.
研究的目的:
- 开发一种新的单元光遗传工具,用于酵母中诱导基因调节.
- 设计一个高效的Saccharomyces cerevisiae细胞工厂,用于生产patchoulol.
- 应用光遗传控制来实现动态代谢流量重定向.
主要方法:
- 与CRISPR-dCas9.9集成的iLight9光遗传生物传感器的开发.
- 使用蛋白质降解标签增强系统稳定性,创建iLight9O.
- 在Saccharomyces cerevisiae的新陈代谢工程中用于patchoulol生物合成,包括异二醇利用途径.
- 使用iLight9O系统对烯合成酶的光遗传动态下调.
主要成果:
- 通过特定的sgRNAs,iLight9O系统提供了可编程的基因调节.
- 工程酵母菌株显示了增强的帕丘洛尔生物合成能力.
- 斯卡伦合成酶的光遗传性下调增加了一种菌株的patchoulol标位66%,另一种菌株的24%.
- 光遗传学方法的性能优于静态工程方法.
结论:
- iLight9O系统提供了一个多功能平台,用于在酵母中精确,光感应的基因调节.
- 代谢途径的动态光遗传控制可以显著提高像patchoulol这样有价值的化合物的产生.
- 这项研究提出了一种通过光遗传流量控制优化微生物细胞工厂的新策略.
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