西诺因通过调节IL-6基因促进物甲基化来治疗类风湿性关节炎
Jin-Fang Luo1, Yang Yu1, Yun-Da Yao2
1Department of Basic Medicine, Department of Pharmacy, Key Laboratory on the Property & Effect of Chinese Medicine (Ethnic Medicine), The Second Clinical Medical School, Guizhou Genuine Herbs Center of Consistency of Utility, The Provincial Key Miao Medicine Laboratory of Guizhou, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, PR China.
概括
西诺因 (SIN) 通过表观遗传性增加介素-6 (IL-6) 基因促进物甲基化来减少炎症和类风湿性关节炎 (RA). 这种表观遗传调节调节炎症反应,为RA提供了一种新的治疗机制.
科学领域:
- 药理学和免疫学 药理学和免疫学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 传统中国医药 传统中国医药
背景情况:
- 来自Sinomenium acutum的Sinomenine (SIN) 显示了对类风湿性关节炎 (RA) 的治疗潜力.
- 在SIN的抗RA作用的基础上,精确的分子机制尚未完全阐明.
- 了解SIN的机制对于优化其在RA治疗中的使用至关重要.
研究的目的:
- 研究SIN.的抗炎和抗RA机制.
- 为了探索SIN对IN-6 (IL-6) 基因促进剂甲基化的影响.
- 整合网络药理学和实验验证,以发现机制.
主要方法:
- 网络药理学和生物信息学确定了RA中SIN的关键目标和途径.
- 分子对接评估了SIN与已识别的目标的结合亲和力.
- 在体外研究中使用LPS刺激的细胞来评估炎症标记物和IL-6促进物甲基化.
- 在体内研究中使用了原诱导性关节炎 (CIA) 鼠标模型来评估治疗疗效.
主要成果:
- SIN主要针对TNF-α,IL-1β,IL-6,STAT3,PTGS2和JAK2,主要通过JAK-STAT通路.
- 在体外,SIN抑制了LPS诱导的炎症,并增加了IL-6促进物甲基化.
- 在体内,SIN治疗减少了CIA大鼠的脚胀,关节炎的严重程度和炎症性细胞因子水平.
结论:
- 辛诺因通过对IL-6基因促进体的表观遗传修饰来缓解RA进展和炎症.
- 通过SIN增强IL-6促进体甲基化是调节炎症反应的关键机制.
- 这项研究强调了SIN作为RA的表观遗传治疗剂的潜力.
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