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Updated: Feb 14, 2026
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Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
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在口腔癌中表皮层-介质细胞过渡,免疫反应和细胞外矩阵重塑
Nicole Lonni1, Camilla Kammer Pereira1, Amanda Maciel Costa1
1Health Sciences Center, Federal University of Santa Catarina, SC, Brazil.
概括
口腔状细胞癌 (OSCC) 中的癌症相关纤维细胞 (CAF) 与侵入性瘤表型和改变的免疫细胞透有关. 这些发现表明,CAFs调节瘤微环境,影响患者的生存.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 癌症生物学 癌症生物学
背景情况:
- 口腔状细胞癌 (OSCC) 的特点是复杂的瘤微环境 (TME).
- 表皮-介质细胞转换 (EMT) 是癌症进展中的一个关键过程.
- 在OSCC中,EMT和免疫反应之间的相互作用仍然不完全理解.
研究的目的:
- 调查EMT标记物和OSCC TME中的免疫细胞透之间的关系.
- 探索癌症相关纤维细胞 (CAFs) 在调节瘤微环境和侵入性表型中的作用.
主要方法:
- 用免疫组织化学方法对OSCC标本进行回顾性分析.
- 对EMT标记物 (PDP,CDH1,VIM,FN,TNC,VEGF) 和CAF标记物 (α-SMA) 的评估.
- 免疫细胞的量化 (中性粒细胞通过CD66b,T淋巴细胞通过CD8).
主要成果:
- 类似EMT的表型 (减少CDH1,增加VIM,PDP) 与CAF激活 (α-SMA) 和VEGF表达相关.
- 增加的VEGF与增加的瘤入侵有关.
- 增加的中性粒细胞 (CD66b) 和减少的CD8+ T细胞与特定的EMT和CAF标记物 (TNC,FN) 相关.
- 较高的中性粒细胞数量和中性粒细胞与淋巴细胞的比率与较差的整体存活率相关.
结论:
- CAF与EMT和侵入性的OSCC表型有关.
- 在TME中,CAF可能在免疫调节中发挥作用.
- 这些发现突出了CAF作为OSCC的潜在治疗点.
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