纳米颗粒和胺补充剂对冷保存的雄羊精子质量,氧化状态和PRDX5基因表达的影响
Cumali Kaya1, Cansu Can2, Burcu Esin1
1Department of Animal Reproduction and Artificial Insemination, University of Ondokuz Mayis, 55200 Samsun, Türkiye.
Animals : an open access journal from MDPI
|February 13, 2026
概括
低剂量的纳米粒子 (SeNPs) 在冷保存后显著提高雄羊精液质量. 这项研究表明,SeNP可以提高精子的运动性,活力和形态,而不会改变氧化状态或PRDX5基因表达.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 生物技术是生物技术.
背景情况:
- 雄羊精液的冷保存对于辅助生殖技术至关重要.
- 冷期间的氧化应激会对精子质量产生负面影响.
- 和纳米粒子 (SeNPs) 正在研究它们的抗氧化特性.
研究的目的:
- 为了评估 (Se) 和SeNP对解后雄羊精子质量的影响.
- 为了评估PRDX5基因表达和氧化状态,冷保存的公羊精液补充Se或SeNPs.
- 确定SeNP的最佳剂量,以改善冷保存的公羊精液参数.
主要方法:
- 来自五只公羊的精液样本使用具有不同度Se或SeNPs (1μg/mL,10μg/mL) 的扩展器进行了冷保存.
- 经解后的精子质量使用计算机辅助精子分析 (CASA) 来评估,包括运动性,活力,膜完整性 (HOST),染色质凝结和形态学.
- 分析了总氧化剂状态 (TOS) 和PRDX5基因表达.
主要成果:
- 与对照组和高剂量Se组相比,低剂量SeNP (1μg/mL) 显著改善了总和渐进性运动,活力,HOST阳性精子和形态正常的精子 (p <0.05).
- 接受SeNP治疗的组表现出最小的染色质凝结异常.
- 两组之间没有观察到TOS或PRDX5基因表达的显著差异 (p > 0.05).
结论:
- 低剂量的SeNP (1μg/mL) 有效地增强雄羊精子解后的功能完整性和冷耐受性.
- SeNP可以改善精子的关键参数,而不会对氧化状态或PRDX5基因表达产生负面影响.
- 纳米粒子是改善公羊精液冷保存结果的有希望的补充.
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