同时改变DNA甲基化和RNAm6A甲基化在表观遗传和转录基因重编程期间诱导的尾巴对接压力在脂肪尾巴绵羊
Jian Zhang1, Yannan Ma2, Shuzhen Song1
1Animal Husbandry, Pasture and Green Agriculture Institute, Gansu Academy of Agricultural Science, Lanzhou 730070, China.
Animals : an open access journal from MDPI
|February 13, 2026
概括
绵羊的尾巴对接通过改变DNA甲基化和RNA m6A修饰来表观遗传学地重编程基因表达,影响组织发育和应激反应,以改善牲畜特征.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 动物科学动物科学
- 分子生物学分子生物学
背景情况:
- 尾巴对接是一种常见的做法,用于肥尾羊的畜牧业.
- 它影响尾巴脂肪沉积,生产性能和动物健康.
- 了解这些影响背后的分子机制至关重要.
研究的目的:
- 为了研究尾巴对接对肥尾羊的基因组和转录组的表观遗传重编程效应.
- 整合DNA甲基化和RNA m6A修饰数据以了解基因表达调节.
- 为了确定关键的基因和途径,涉及到尾部对接的反应.
主要方法:
- 全基因组二硫酸盐测序 (WGBS) 用于分析DNA甲基化模式.
- RNA m6A甲基化免疫沉降测序 (MeRIP-seq) 来映射RNA m6A的修改.
- 综合分析WGBS和MeRIP-seq数据,并使用热测序和qPCR进行验证.
主要成果:
- 尾部对接导致了广泛的DNA低甲基化和增加的RNA m6A修饰.
- 不同的甲基化区域被丰富了像Hippo信号和粘附结这样的途径.
- 综合分析确定了具有同时DNA和RNA甲基化变化的基因,特别是CITED4和ZNF644.4.
结论:
- 尾部对接会诱导协调的DNA低甲基化和RNA m6A高甲基化.
- 这些表观遗传变化驱动了对环境干预的转录基因重编程.
- 这些发现为动物特征形成的分子基础提供了新的见解.
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