在Verbascoside中使用经过验证的UHPLC-PDA方法标准化罗马尼亚Galeopsis tetrahit的叶子提取物
Roxana Maria Golu1,2, Ludovic Everard Bejenaru3,4, Andrei Biţă3,4
1Doctoral School, University of Medicine and Pharmacy of Craiova, 2 Petru Rareş Street, 200349 Craiova, Romania.
Plants (Basel, Switzerland)
|February 13, 2026
概括
这项研究将Galeopsis tetrahit L.叶片提取物标准化为Verbascoside,这是一个关键的抗氧化化合物. 一种经过验证的UHPLC-PDA方法证实了提取物质量和抗氧化能力,为这种传统欧洲药物物种建立了框架.
科学领域:
- 药物鉴定和植物化学学
- 分析化学 分析化学
- 药用植物研究 药用植物研究
背景情况:
- (Lamiaceae) 是一种传统的欧洲药用植物.
- 它含有丰富的化合物,特别是维巴斯科赛德,以其抗氧化特性而闻名.
- 标准化对于确保草本提取物的质量和有效性至关重要.
研究的目的:
- 根据其verbascoside含量来标准化G. tetrahit叶片提取物.
- 开发和验证一种UHPLC-PDA方法来定量维巴斯科赛德.
- 评估提取物及其主要生物活性化合物的抗氧化潜力.
主要方法:
- 来自罗马尼亚的野生生长的G. tetrahit的叶子用70%的乙醇提取.
- 一种UHPLC-PDA方法是根据ICH Q2和R2指南开发和验证的.
- 使用保留时间,UV-PDA光谱和QDa质谱证实了维巴斯科西德的鉴定.
- 使用DPPH,ABTS和FRAP测定,以及TPC和TFC测量,评估了抗氧化活性.
主要成果:
- 提取的结果是17.28%的干取物.
- 经过验证的UHPLC-PDA方法显示了高特异性,线性 (r=0.999955) 和低检测/量化极限.
- 干燥提取物每克含有345.8±28.3毫克维巴斯科赛德 (34.6%重/重).
- G. tetrahit 提取物表现出显著的抗氧化活性,其中verbascoside 是主要的贡献者.
结论:
- 建立了一种可靠的UHPLC-PDA分析方法,用于在G. tetrahit提取物中标准化verbascoside.
- 该研究为罗马尼亚原产的G. tetrahit提取物提供了一个基于verbascoside的标准化框架.
- 这些发现支持了G. tetrahit的传统使用,并强调了作为其抗氧化潜力的标记物维巴斯科赛德的重要性.
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