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从红藻Asparagopsis taxiformis中超声波辅助提取多糖:RAW264.7细胞中的优化,特征,机制和免疫活动
Kun Yang1,2, Yuxin Wang2, Wentao Zou2
1Shenzhen Research Institute, Guangdong Ocean University, Shenzhen 518108, China.
Foods (Basel, Switzerland)
|February 13, 2026
概括
深度浸泡溶剂 (DES) 是从红藻中提取多糖的可持续方法,产量是提取水的1.5倍. 然而,DES提取可能会减少多糖.
科学领域:
- 海洋生物技术 海洋生物技术
- 自然产品化学 自然产品化学
- 生物化学 生物化学
背景情况:
- 传统的多糖提取方法效率低下,耗费大量能量.
- 深度环氧溶剂 (DES) 为生物质加工提供了一个可持续的替代方案.
- 红藻如 *A. taxiformis* 是富含生物活性多糖的丰富来源.
研究的目的:
- 为了优化DES辅助的超声波提取多糖从*A. taxiformis*.
- 描述提取的多糖,并将它们的结构和免疫特性与水提取的对应物进行比较.
- 阐明DES提取对多糖的结构-活性关系的影响.
主要方法:
- 使用响应表面方法的优化DES (ChCl-LA) 辅助超声波提取.
- 结构特征包括单糖化合物组成和分子量测定.
- 通过巨细胞消化和细胞因子分泌测试进行免疫活性评估.
- 计算模拟 (DFT,MD) 用于研究溶剂-多糖相互作用.
主要成果:
- 优化的DES提取实现了11.28%的收益率,比水提取高1.5倍.
- 通过DES提取的多糖是酸性,主要由银河糖组成,分子量为99.88kDa.
- 与提取水相比,DES提取显著降低了免疫活性,这可能是由于化学配置的改变.
结论:
- 胆酸-乳酸 (ChCl-LA) DES有效地从*A. taxiformis*中提取高产多糖.
- 提取方法显著影响多糖体结构,分子量和免疫功能.
- 了解这些结构-活性关系对于在功能性食品中高价值的藻类多糖的利用至关重要.
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