用Bacillus natto发酵小麦的过程分析:酶活动和生物活性成分的动态变化
Shimei Zhang1, Fanqiang Meng1, Xia Fan1
1College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Foods (Basel, Switzerland)
|February 13, 2026
概括
在48-60小时内用Bacillus natto发酵小米,可优化微生物生长,酶活性和可溶性食纤维和类生物活性成分. 这增强了小米的功效.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 小米是丰富的生物活性化合物的来源,但需要加工以提高利用率.
- 使用像Bacillus natto这样的微生物发酵可以将农业副产品转化为增值产品.
研究的目的:
- 为了确定小米的最佳发酵时间,使用Bacillus natto.
- 分析微生物生长,酶活动和发酵期间生物活性成分的动态变化.
主要方法:
- 监测活跃的细菌和子数量超过84小时.
- 测量蛋白质酶,氨基酶,细胞酶和纳托基纳斯 (NK) 的纤维分解活性.
- 量化总糖,减少糖,可溶性食纤维 (SDF),β-葡萄糖,阿拉比诺基兰,和多.
主要成果:
- 细菌数量在48小时达到峰值,而子数量增加了84小时.
- 酶活性 (蛋白酶,氨基酶,细胞酶,NK) 在48小时内达到最大.
- SDF,和多显著增加,在60小时达到峰值;β-葡萄糖和阿拉比诺基兰也在特定时间点显著增加.
结论:
- 使用Bacillus natto的小米的最佳发酵时间为48-60小时.
- 发酵显著提高了关键生物活性成分和酶活动的含量.
- 这项研究为在功能性食品开发中高价值的小米的利用提供了基础.
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