通过结合等离子体功能化液体和超声波来实现持久的带净化和可持续的干细胞采购
Yuanyuan Pan1, Alexander Pogoda1, Monika Röntgen2
1Department of Plasma Life Science, Leibniz Institute for Plasma Science and Technology e.V. (INP), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
Foods (Basel, Switzerland)
|February 13, 2026
概括
血功能化液体与超声波相结合,有效地去污染猪带,用于基于细胞的肉类生产. 这种无抗生素的方法保持了间酶体干细胞的产量和活力,使得可持续的采购成为可能.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 食品科学 食品科学 食品科学
背景情况:
- 对替代肉类需求的不断增长推动了对基于细胞的肉类生产的研究.
- 猪带组织是培养肉类中介细胞干细胞 (MSC) 的有希望的来源.
- 组织的有效去污染是至关重要的,但在不损害细胞完整性的情况下具有挑战性.
研究的目的:
- 评估血功能化液 (PFL) 用于清除猪带的污染.
- 评估单独和超声波的PFL对微生物减少的有效性.
- 为了确定PFL治疗对MSC产量,生存能力和功能的影响.
主要方法:
- 猪的带被微波驱动的等离子源产生的PFL处理.
- 治疗包括单独的PFL和与超声波结合的PFL.
- 评估了微生物负载减少,MSC产量,细胞活力和3D球形形成.
主要成果:
- 超声波和PFL组合显著降低了微生物负载 (4.087.41 log10 CFU/g),接近检测极限.
- MSC产量 (5.4 × 105细胞/g) 和活力 (84%) 与抗生素治疗对照组相比较.
- 恢复的MSC保持了功能能力,形成3D球形.
结论:
- 超声波辅助的PFL冲洗是一种有效的,无抗生素的猪带污染消除策略.
- 这种方法与组织完整性兼容,并保留培养肉类应用的干细胞质量.
- 这项研究证明了可持续的干细胞采购,并扩大了PFL在生物处理中的应用.
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