多态和分子对接显示,麦和麦可以通过IL-17路径调节来缓解慢性结肠炎
Wen Duan1,2, Tong Li3, Yuyu Zhang1,2
1Key Laboratory of Geriatric Nutrition and Health, Beijing Technology and Business University, Ministry of Education, Beijing 100048, China.
麦和麦可以通过调节IL-17信号通路并增强肠道屏障功能来缓解慢性结肠炎. 这些饮食干预措施影响肠道微生物群和关键代谢物,为炎症性肠病提供治疗潜力.
科学领域:
- 胃肠病学 胃肠病学
- 营养科学 营养科学
- 免疫学 免疫学 免疫学
背景情况:
- 饮食显著影响炎症性肠病 (IBD) 的发展.
- 以前的研究表明,麦和麦可以通过肠道微生物群调节在小鼠中减轻酸 (DSS) 诱导的结肠炎.
研究的目的:
- 阐明整个麦和麦干预措施影响慢性结肠炎的机制.
- 用综合代谢和转录组分析来比较麦和麦的影响.
主要方法:
- 组合的非目标代谢学和转录学分析.
- 使用分子对接,研究了关键代谢物 (ursodeoxycholic 酸,3-(3-hydroxyphenyl) propionic 酸, avenanthramide C) 和 IL-17 信号通路蛋白之间的相互作用.
- 通过RT-qPCR分析基因表达.
主要成果:
- 确定了参与大肠炎调节的三个关键代谢物.
- 麦和麦干预改变了IL-17,PI3K-Akt和TNF信号通路.
- 调紧结蛋白 (克劳丁-1,克劳丁-5,奥克卢丁) 和减少炎症标志物.
- 乌尔索多西胆酸表现出对目标蛋白质的结合亲和力最高.
结论:
- 麦和麦可以缓解慢性结肠炎.
- 调节IL-17信号通路和增强肠道屏障功能是关键机制.
- 用麦进行的饮食干预措施在治疗IBD方面表现有前途.
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